RPS21 Overexpression Lysate

Western Blot: RPS21 Overexpression Lysate (Adult Normal) [NBP2-08653] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RPS21.

• Reactivity: Hu
• Applications: WB

RPS21 Overexpression Lysate Summary

• Description:

  RPS21 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC217512
  
  Accession#: NM_001024
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: RPS21

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 8.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for RPS21 Overexpression Lysate

• ribosomal protein S21,40S ribosomal protein S21,8.2 kDa differentiation factor

Background

RPS21 is a gene that codes for a ribosomal protein that is a component of the 40S subunit, and has a length of 83 amino acids and a weight of approximately 9 kDa. Current studies are being done on several disorders and diseases related to this gene, including pure red-cell aplasia, anemia, malaria, and leukemia. RPS21 has also been shown to have interactions with RIOK2, RPSA, ABCF2, KRR1, and MCTS1 in pathways such as the cytoplasmic ribosomal protein, PB1-F2 synthesis, influenza life cycle, and viral mRNA translation pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: RPS21