RPRM Overexpression Lysate

Images

 
Western Blot: RPRM Overexpression Lysate (Adult Normal) [NBL1-15556] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RPRM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RPRM Overexpression Lysate Summary

Description

RPRM Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202634

Accession#: NM_019845

Protein Tag: C-MYC/DDK
Gene
RPRM

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
11.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RPRM Overexpression Lysate

  • candidate mediator of the p53 dependent G2 arrest
  • candidate mediator of the p53-dependent G2 arrest
  • FLJ90327
  • protein reprimo
  • REPRIMO
  • reprimo, TP53 dependant G2 arrest mediator candidate
  • reprimo, TP53 dependent G2 arrest mediator candidate

Background

RPRM may be involved in the regulation of p53-dependent G2 arrest of the cell cycle. Seems to induce cell cycle arrest by inhibiting CDC2 activity and nuclear translocation ofthe CDC2 cyclin B1 complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol RPRM