RPL7A Antibody - BSA Free

Western Blot: RPL7A Antibody [NBP2-20217] - 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE RPL7A antibody dilution: 1:1000

Immunocytochemistry/Immunofluorescence: RPL7A Antibody [NBP2-20217] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: RPL7A Antibody [NBP2-20217] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:500 dilution.

Western Blot: RPL7A Antibody [NBP2-20217] - Sample (30 ug of whole cell lysate) A: A431 B: JurKat C: Raji 12% SDS PAGE gel, diluted at 1:1000.

Western Blot: RPL7A Antibody [NBP2-20217] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 12% SDS PAGE gel, diluted at 1:3000.

Immunohistochemistry: RPL7A Antibody [NBP2-20217] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RPL7A antibody (NBP2-20217) at 1:300 dilution.

Western Blot: RPL7A Antibody [NBP2-20217] - Sample (30 ug of whole cell lysate) A: whole zebrafish B: zebrafish eye 12% SDS PAGE NBP2-20217 diluted at 1:1000

Western Blot: RPL7A Antibody [NBP2-20217] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPL7A antibody (NBP2-20217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ze, Bv, Ca, Ch, RM, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPL7A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RPL7A. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (100%), Zebrafish (93%), Bovine (99%), Xenopus (93%), Canine (100%), Chicken (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPL7A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (93%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RPL7A Antibody - BSA Free

• 60S ribosomal protein L7a
• PLA-X polypeptide
• ribosomal protein L7a
• SURF-3
• SURF3surfeit 3
• Surfeit locus protein 3
• thyroid hormone receptor uncoupling protein
• TRUP

Background

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RPL7A