RPL11 Antibody - BSA Free

Western Blot: RPL11 Antibody [NBP3-38259] - Western blot analysis of lysates from 293F cells, using RPL11 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: RPL11 Antibody [NBP3-38259] - Immunofluorescence analysis of L929 cells using RPL11 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western Blot: RPL11 Antibody [NBP3-38259] - Western blot analysis of various lysates, using RPL11 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPL11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RPL11 Antibody - BSA Free (NBP3-38259) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of RPL11 (NP_001186731.1).
  
  Sequence:
  MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPL11
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for RPL11 Antibody - BSA Free

• cell growth-inhibiting protein 34,60S ribosomal protein L11
• CLL-associated antigen KW-12
• DBA7
• GIG34
• ribosomal protein L11

Background

RPL11, also known as 60S ribosomal protein L11, is a 20kDa 178 amino acid protein with a shorter 20kDa 177 isoform produced by alternative splicing. RPL11 is required for rRNA maturation and can be found in the nucleus. Current research is being conducted on RPL11 in relation to aplastic anemia, anemia, pneumonia, retinitis, tuberculosis, malaria and mycobacterium tuberculosis. RPL11 has been linked to the cell cycle arrest, cell proliferation, methylation and translation pathways where it interacts with RPS15A, RPS3, RPS2, RPL26 and MDM2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPL11