RNF2 Overexpression Lysate (Native)

Images

 
Western Blot: RNF2 Overexpression Lysate (Adult Normal) [NBL1-15442] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNF2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RNF2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203089 and based on accession number NM_007212. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ring finger protein 2 (RNF2), mRNA.
Gene
RNF2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RNF2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RNF2 Overexpression Lysate (Native)

  • BAP-1
  • BAP1RING finger protein 1B
  • DING
  • DINGRING finger protein BAP-1
  • EC 6.3.2
  • HIPI3
  • HIPI3RING1BE3 ubiquitin-protein ligase RING2
  • Huntingtin-interacting protein 2-interacting protein 3
  • Protein DinG
  • ring finger protein 2HIP2-interacting protein 3
  • RING1B
  • RING2
  • RING2EC 6.3.2.-
  • RNF2

Background

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for RNF2 Lysate (NBL1-15442) (0)

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Bioinformatics

Gene Symbol RNF2
Entrez