RNF2 Antibody [Allophycocyanin]

• Reactivity: Hu, Mu, ChHa
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

RNF2 Antibody [Allophycocyanin] Summary

• Immunogen: RNF2 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RNF2 protein. (Uniprot: Q99496)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RNF2 from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RNF2 homologues from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RNF2 Antibody [Allophycocyanin]

• BAP-1
• BAP1RING finger protein 1B
• DING
• DINGRING finger protein BAP-1
• EC 6.3.2
• HIPI3
• HIPI3RING1BE3 ubiquitin-protein ligase RING2
• Huntingtin-interacting protein 2-interacting protein 3
• Protein DinG
• ring finger protein 2HIP2-interacting protein 3
• RING1B
• RING2
• RING2EC 6.3.2.-
• RNF2

Background

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF2