RNF139 Antibody

Western Blot: RNF139 Antibody [NBP1-23027] - (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: RNF139 Antibody [NBP1-23027] - (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RNF139 Antibody Summary

• Immunogen: Peptide with sequence C-HTGAAAEEFNDDT corresponding to C-Terminus according to NP_009149.2.
• Epitope: internal region
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNF139
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 4 ug/ml
  • Immunohistochemistry-Paraffin 3 - 4 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in human skeletal muscle lysates (calculated MW of 76.0 kDa band according to NP_009149.2). IHC-P: Human placenta shows membranous staining in the cytotrophoblasts.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNF139 Antibody

• EC 6.3.2.-
• HRCA1
• patched related protein translocated in renal cancer
• RCA1multiple membrane spanning receptor TRC8
• ring finger protein 139MGC31961
• Translocation in renal carcinoma on chromosome 8 protein
• TRC8E3 ubiquitin-protein ligase RNF139

Background

The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF139
• Entrez:
  • 11236(Human)
• Uniprot:
  • NP_009149.2(Human)