RLBP1L1 Antibody - Azide and BSA Free

Western Blot: RLBP1L1 Antibody [NBP2-29652] - rat hippocampal homogenate showing specific immunolabeling of the ~ 35k clavesin protein.

• Reactivity: Rt, Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RLBP1L1 Antibody - Azide and BSA Free Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # A6JFQ6
• Specificity: Specific for endogenous levels of the ~35 kDa clavesin 1/2 protein doublet. Isoform-specific knock down in cultured hippocampal neurons indicates that the lower and upper bands are clavesin 1 and 2, respectively.
• Preparation Method: Prepared from serum by affinity purification using a column to which the peptide antigen was coupled.
• Predicted Species: Human (100%), Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLVS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is anticipated that the antibody will also work in human and mouse as there is 100% homology in these species with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: No Preservative
• Purity: Affinity purified

Alternate Names for RLBP1L1 Antibody - Azide and BSA Free

• Cellular retinaldehyde-binding protein-like
• clavesin 1
• clavesin-1
• CRALBPLC6orf212L
• FLJ37248
• MGC34646
• retinaldehyde binding protein 1-like 1
• Retinaldehyde-binding protein 1-like 1
• RLBP1L1

Background

Clavesin (clathrin vesicle associated Sec14 protein) is a novel neuron specific protein that has recently been identified and shown to be required for normal morphology of late endosomes and/or lysosomes as lentiviral-mediated knockdown of clavesin in hippocampal neurons causes lysosomal defects (Katoh et al., 2009). Additionally, upregulation of clavesin in human hepatocellular carcinoma has recently been demonstrated thus making it a useful marker for this disease state (Zhao et al., 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLVS1
• Entrez:
  • 366311(Rat)
• Uniprot:
  • A6JFQ6(Rat)