RIOK2 Antibody (OTI3E11) - Azide and BSA Free

Western Blot: RIOK2 Antibody (OTI3E11) - Azide and BSA Free [NBP2-73910] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIOK2.

Immunocytochemistry/Immunofluorescence: RIOK2 Antibody (OTI3E11) - Azide and BSA Free [NBP2-73910] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RIOK2.

Immunohistochemistry: RIOK2 Antibody (OTI3E11) - Azide and BSA Free [NBP2-73910] - Analysis of Human pancreas tissue.

Western Blot: RIOK2 Antibody (OTI3E11) - Azide and BSA Free [NBP2-73910] - Analysis of extracts (35ug) from 9 different cell lines by using g RIOK2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: RIOK2 Antibody (OTI3E11) - Azide and BSA Free [NBP2-73910] - Analysis of Adenocarcinoma of Human breast tissue.

Immunohistochemistry: RIOK2 Antibody (OTI3E11) - Azide and BSA Free [NBP2-73910] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: RIOK2 Antibody (OTI3E11) - Azide and BSA Free [NBP2-73910] - Analysis of Human bladder tissue.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI3E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RIOK2 Antibody (OTI3E11) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 277-552 of human RIOK2(NP_060813) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RIOK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-4000
• Theoretical MW: 63.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RIOK2 Antibody (OTI3E11) - Azide and BSA Free

• EC 2.7.11.1
• FLJ11159
• RIO kinase 2 (yeast)
• RIO kinase 2
• RIO2
• RIOK2
• serine/threonine-protein kinase RIO2

Background

RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIOK2