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RIC3 Antibody

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 (1 Review)
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Western Blot: RIC3 Antibody [H00079608-B01P] - Analysis of RIC3 expression in transfected 293T cell line by RIC3 polyclonal antibody. Lane 1: RIC3 transfected lysate(40.48 KDa). Lane 2: Non-transfected lysate.
Western Blot: RIC3 Antibody [H00079608-B01P] - Analysis of RIC3 expression in transfected 293T cell line by RIC3 polyclonal antibody. Lane 1: RIC3 transfected lysate(40.48 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity Hu, ChHa, XpSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

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RIC3 Antibody Summary

Immunogen
RIC3 (NP_078833.2, 1 a.a. - 368 a.a.) full-length human protein. MAYSTVQRVALASGLVLALSLLLPKAFLSRGKRQEPPPTPEGKLGRFPPMMHHHQAHSDGQTPGARFQRSHLAEAFAKAKGSGGGAGGGGSGRGLMGQIIPIYGFGIFLYILYILFKLSKGKTTAEDGKCYTAMPGNTHRKITSFELAQLQEKLKETEAAMEKLFNRVGPNGERAQTVTSDQEKRLLHQLREITRVMKEGKFIDRFSPEKEAEEAPYMEDWEGYPEETYPIYDLSDCIKRRQETILVDYPDPKELSAEEIAERMGMIEEEESDHLGWESLPTDPRAQEDNSVTSCDPKPETCSCCFHEDEDPAVLAENAGFSADSYPEQEETTKEEWSQDFKDEGLGISTDKAYTGSMLRKRNPQGLE
Specificity
Reacts with resistance to inhibitors of cholinesterase 3 homolog (C. elegans).
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
RIC3
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This antibody is reactive against transfected lysate in western blot.
Reviewed Applications
Read 1 Review rated 3
using
H00079608-B01P in the following application:
Publications
Read Publications using
H00079608-B01P in the following applications:

  • WB
    1 publication

Reactivity Notes

Customer Review indicates reactivity in both CHO K1 Cell lines and Frog oocytes.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RIC3 Antibody

  • AYST720
  • FLJ11608
  • PRO1385
  • protein RIC-3
  • resistance to inhibitors of cholinesterase 3 homolog (C. elegans)

Background

RIC3 is a protein associated with nicotinic acetylcholine receptors (nAChRs), neurotransmitter-gated ion channels expressed at the neuromuscular junction and within the central and peripheral nervous systems, that can enhance functional expression of multiple nAChR subtypes (Lansdell et al., 2005 [PubMed 16120769]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(1 Review)
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Publications for RIC3 Antibody (H00079608-B01P)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for RIC3 Antibody (H00079608-B01P) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using H00079608-B01P:
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
 WB Other 03/22/2010
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Summary

ApplicationWestern Blot
Sample TestedCHO K1 Cell lines/ Frog oocytes
SpeciesOther
Lot09135WULz

Blocking

Blocking DetailsBlocking Buffer: 5% Milk (non-fat), Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/500, Incubation Dilution Buffer: 5% milk, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Thermo Scientific Goat Anti-Mouse IgG (H+L), Secondary Ab Dilution Ratio: 1/50000

Details

Detection NotesMethod:ECL, Time:20min, NegCtrl:Mammalian nontransfected cells/Uninjected oocytes, Band:60kDa(specific), 100kDa(non-specific)

Product General Protocols

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Video Protocols

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FAQs for RIC3 Antibody (H00079608-B01P) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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 Verified Customer
03/22/2010
Application: WB
Species: Other
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Bioinformatics

Gene Symbol RIC3
Entrez
Uniprot