Ribosomal Protein S6/RPS6 Antibody [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Ribosomal Protein S6/RPS6 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Ribosomal Protein S6
  Met1-Lys249
  Accession # P62753
• Specificity: Detects human, mouse and rat Ribosomal Protein S6 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ribosomal Protein S6/RPS6 Antibody [Alexa Fluor® 350]

• Phosphoprotein NP33, 40S ribosomal protein S6
• pS6
• Ribosomal Protein S6
• RPS6
• S6

Background

(40S) Ribosomal protein S6 (rpS6; also Phosphoprotein NP33) is a 32-34 kDa member of the ribsosmal protein S6e family of molecules. It is ubiquitously expressed and involved in the regulation of cell size. Higher eukaryotic ribosomes contain a small (or 40S) and large (or 60S) subunit. The small subunits are composed of one RNA molecule plus more than 30 individual proteins, one of which is rp6S. It is a substrate of both PASKIN and S6 kinase, and when phosphorylated C-terminally, appears to slow the rate of protein synthesis. Notably, the activities of rpS6 are complex, as phosphorylation seems to decrease cell proliferation and glucose utilization on one hand, while increasing cell size on the other. Human ribosomal protein S6 is 249 amino acids (aa) in length. It possesses two acetylated lysines plus six phosphorylated serines. There are three potential splice forms. One contains a four aa substitution for aa 1-46, a second utilizes an alternative start site at Met32, and a third utilizes the same Met32 start site coupled to a substitution of a Gln residue for aa 234-243. Full-length human, mouse and rat rpS6 are identical in their amino acid sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.