Ribophorin II Antibody

Western Blot: Ribophorin II Antibody [27500002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 74, 71, 69, 68, 56, 33, 30 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

ELISA: Ribophorin II Antibody [27500002]

Product Discontinued

Ribophorin II Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein.
• Specificity: This product is specific for Human RPN2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: Use in Western Blot reported in scientific literature (PMID:32848200)

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID:32848200).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Ribophorin II Antibody

• RIBIIREC 2.4.1.119
• ribophorin IIDolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
• ribophorin-2
• RPNII
• RPN-IIdolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
• SWP1

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ribophorin II Antibody (27500002)(3)

Publications using 27500002

• Metwally E, Zhao G, Wang Q, Zhang YQ Ttm50 facilitates calpain activation by anchoring it to calcium stores and increasing its sensitivity to calcium Cell Res. 2020-08-26 [PMID: 32848200] (WB, Drosophila)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: RPN2
• Entrez:
  • 6185(Human)
• Uniprot:
  • P04844(Human)