Recombinant Human RhoV His Protein

SDS-Page: RhoV Protein [NBP2-23400]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human RhoV His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-236 of Human RhoV
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPPRELS EAEPPPLRAP TPPPRRRSAP PELGIKCVLV GDGAVGKSSL IVSYTCNGYP ARYRPTALDT FSVQVLVDGA PVRIELWDTA GQEDFDRLRS LCYPDTDVFL ACFSVVQPSS FQNITEKWLP EIRTHNPQAP VLLVGTQADL RDDVNVLIQL DQGGREGPVP QPQAQGLAEK IRACCYLECS ALTQKNLKEV FDSAILSAIE HKARLEKKLN AKGVRTLSRC RWKKFFCFV

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: RHOV
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 28.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human RhoV His Protein

• ARHV
• CDC42-like GTPase 2
• CHP
• GTP-binding protein-like 2
• ras homolog gene family, member V
• Rho GTPase-like protein ARHV
• rho-related GTP-binding protein RhoV
• Wnt-1 regulated Cdc42 homolog 2
• Wnt-1 responsive Cdc42 homolog 2
• WRCH1-related GTPase
• WRCH-2
• WRCH2Chp

Background

Rho-related GTP-binding protein RhoV, also known as RHOV, is a member of the Rho family and small GTPase superfamily. RHOV is a 236 amino acid protein that controls the actin cytoskeleton through activation of the JNK pathway. RHOV functions as a lipid anchor at the cytoplasmic side of the cell membrane and is expressed in placenta, pancreas and fetal brain. Recombinant human RHOV protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RHOV