RhoC Antibody (2E12) - Azide and BSA Free

Western Blot: RhoC Antibody (2E12) [H00000389-M01] - Analysis of RHOC expression in transfected 293T cell line by RHOC monoclonal antibody (M01), clone 2E12.Lane 1: RHOC transfected lysate(22 KDa).Lane 2: Non-transfected lysate.

Western Blot: RhoC Antibody (2E12) [H00000389-M01] - RHOC monoclonal antibody (M01), clone 2E12. Analysis of RHOC expression in PC-12.

Western Blot: RhoC Antibody (2E12) [H00000389-M01] - RHOC monoclonal antibody (M01), clone 2E12 Analysis of RHOC expression in Jurkat.

Western Blot: RhoC Antibody (2E12) [H00000389-M01] - RHOC monoclonal antibody (M01), clone 2E12. Analysis of RHOC expression in NIH/3T3.

Sandwich ELISA: RhoC Antibody (2E12) [H00000389-M01] - Detection limit for recombinant GST tagged RHOC is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 2E12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RhoC Antibody (2E12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RHOC (AAH07245, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQVRKNKRRRGCPIL
• Specificity: RHOC (2E12)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHOC
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RhoC Antibody (2E12) - Azide and BSA Free

• ARH9ARHCRho cDNA clone 9
• H9
• MGC1448
• MGC61427
• oncogene RHO H9
• ras homolog gene family, member C
• RAS-related homolog 9
• rhoC GTPase
• RhoC
• RHOH9
• rho-related GTP-binding protein RhoC
• small GTP binding protein RhoC

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RhoC Antibody (H00000389-M01)(1)

Publication using H00000389-M01

• Han J, Li L, Hu J et al. Epidermal growth factor stimulates human trophoblast cell migration through Rho A and Rho C activation. Endocrinology;151(4):1732-42. 2010-04-01 [PMID: 20150581]

Bioinformatics

• Gene Symbol: RHOC
• Entrez:
  • 389(Human)
  • 11853(Mouse)
  • 295342(Rat)
• OMIM:
  • 165380(Human)
• Uniprot:
  • P08134(Human)
  • Q62159(Mouse)