Rheb Antibody (JG37-12)

Images

 
Western Blot: Rheb Antibody (JG37-12) [NBP2-75646] - Analysis of Rheb on mouse placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Immunocytochemistry/ Immunofluorescence: Rheb Antibody (JG37-12) [NBP2-75646] - Staining RHEB in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Rheb Antibody (JG37-12) [NBP2-75646] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RHEB antibody. Counter stained with hematoxylin.
Flow Cytometry: Rheb Antibody (JG37-12) [NBP2-75646] - Analysis of A431 cells with RHEB antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; ...read more
Immunocytochemistry/ Immunofluorescence: Rheb Antibody (JG37-12) [NBP2-75646] - Staining RHEB in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Rheb Antibody (JG37-12) [NBP2-75646] - Analysis of paraffin-embedded human liver tissue using anti-RHEB antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Rheb Antibody (JG37-12) [NBP2-75646] - Analysis of paraffin-embedded human colon tissue using anti-RHEB antibody. Counter stained with hematoxylin.
Knockdown Validated: Rheb Antibody (JG37-12) [NBP2-75646] - Analysis of RHEB with anti-RHEB antibody [JG37-12] at 1:1,000 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: RHEB knockdown Raw264.7 whole ...read more

Product Details

Summary
Product Discontinued
View other related Rheb Primary Antibodies

Order Details


    • Catalog Number
      NBP2-75646
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Rheb Antibody (JG37-12) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within Human Rheb aa 21-108 / 184. (SwissProt: Q15382 Human; SwissProt: Q921J2 Mouse)
Localization
Cytoplasm. Golgi apparatus. Endoplasmic reticulum.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RHEB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Knockdown Validated
  • Western Blot 1:500-1:2000
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Rheb Antibody (JG37-12)

  • GTP-binding protein Rheb
  • MGC111559
  • Ras homolog enriched in brainRHEB2Ras homolog enriched in brain 2
  • Rheb
  • Rheb2

Background

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, NULL, WB
NBP2-46234
Species: Hu, Mu, Rt
Applications: IHC, WB
MAB40401
Species: Hu, Mu
Applications: ICC, IHC, WB
NB100-1595
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP1-87799
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB600-1049
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24837
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24503
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB200-157
Species: Hu
Applications: IHC, IHC-P, KO, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP1-44270
Species: Mu, Rt
Applications: EM, ICC/IF, IHC, IHC-P, WB
NBP2-13426
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP1-89865
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-88937
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-46404
Species: Hu, Mu, Rt
Applications: IHC, IHC-P

Publications for Rheb Antibody (NBP2-75646) (0)

There are no publications for Rheb Antibody (NBP2-75646).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Rheb Antibody (NBP2-75646) (0)

There are no reviews for Rheb Antibody (NBP2-75646). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rheb Antibody (NBP2-75646) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Rheb Products

Bioinformatics Tool for Rheb Antibody (NBP2-75646)

Discover related pathways, diseases and genes to Rheb Antibody (NBP2-75646). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rheb Antibody (NBP2-75646)

Discover more about diseases related to Rheb Antibody (NBP2-75646).
 

Pathways for Rheb Antibody (NBP2-75646)

View related products by pathway.

PTMs for Rheb Antibody (NBP2-75646)

Learn more about PTMs related to Rheb Antibody (NBP2-75646).
 

Research Areas for Rheb Antibody (NBP2-75646)

Find related products by research area.

Blogs on Rheb.

TSC1 - a negative regulator of mTOR signaling
TSC1 is a tumor suppressor gene that encodes a 130 kDa protein called hamartin. TSC1 was first identified as an oncogenic driver of Tuberous Sclerosis, a condition characterized by numerous benign tumors of the skin, brain, heart, and lungs. A mut...  Read full blog post.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

Omicron Variant Antibodies

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Rheb Antibody (JG37-12) and receive a gift card or discount.

Bioinformatics

Gene Symbol RHEB