RGS5 Antibody (OTI1C1) - Azide and BSA Free

Western Blot: RGS5 Antibody (OTI1C1) - Azide and BSA Free [NBP2-73887] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.

Immunohistochemistry: RGS5 Antibody (OTI1C1) - Azide and BSA Free [NBP2-73887] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RGS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min)

Flow Cytometry: RGS5 Antibody (OTI1C1) - Azide and BSA Free [NBP2-73887] - Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry: RGS5 Antibody (OTI1C1) - Azide and BSA Free [NBP2-73887] - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RGS5 antibody and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RGS5 Antibody (OTI1C1) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RGS5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 20.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RGS5 Antibody (OTI1C1) - Azide and BSA Free

• MST092
• MST106
• MST129
• MSTP032
• MSTP092
• MSTP106
• MSTP129
• regulator of G-protein signaling 5
• regulator of G-protein signalling 5

Background

Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins are GTPase-activating proteins for Gi and Gq class G-alpha proteins. They accelerate transit through the cycle of GTP binding and hydrolysis and thereby accelerate signaling kinetics and termination. RGS5 seems to be an important signalling regulator in the vascular system. Present data suggest that RGS5 may be involved in the regulation of capillary growth, angiogenesis, and in the pathophysiology of stroke.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RGS5