RGS1 Antibody - BSA Free

Immunohistochemistry-Paraffin: RGS1 Antibody [NBP2-20146] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Western Blot: RGS1 Antibody [NBP2-20146] - Untreated (-) and treated (+) THP-1 whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS1 antibody (NBP2-20146) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RGS1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RGS1. The exact sequence is proprietary.
• Predicted Species: Bovine (90%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RGS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RGS1 Antibody - BSA Free

• 1R20
• 1R20Early response protein 1R20
• B-cell activation protein BL34
• BL34
• Early response protein 1R20
• HEL-S-87
• IER1
• IR20
• IR20immediate-early response 1, B-cell specific
• regulator of G-protein signaling 1
• regulator of G-protein signalling 1
• RGS1

Background

This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RGS1
• Uniprot:
  • Q08116(Human)