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RGL2 Antibody

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Immunoprecipitation: RGL2 Antibody [NBP2-59128] - Detection of human RGL2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RGL2 antibody NBP2-59128 used for IP at 8 ul per reaction. RGL2 was also immunoprecipitated by another rabbit anti-RGL2 antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RGL2 Antibody [NBP2-59128] - Detection of human RGL2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.4 mg/ml

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RGL2 Antibody Summary

Immunogen
Maps to a region between 727 and 777
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RGL2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 8-15 ug/mg lysate
Application Notes
Western Blot Not recommended.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Tris-buffered Saline containing 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RGL2 Antibody

  • GDS-related protein
  • HKE1.5
  • KE1.5
  • RAB2, member RAS oncogene family-like
  • RAB2LRas-associated protein RAB2L
  • ral guanine nucleotide dissociation stimulator-like 2
  • RalGDS-like 2
  • RalGDS-like factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-93790
Immunocytochemistry/Immunofluorescence: RALGDS Antibody [NBP2-93790] - Analysis of L929 cells using RALGDS at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: RALGDS Antibody [NBP2-93790] - Analysis of U-2 OS cells using RALGDS at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RALGDS Antibody [NBP2-93790] - Paraffin-embedded human thyroid cancer using RALGDS .
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
GLS2 Antibody -
BSA Free
NBP1-76544
Western Blot: GLS2 Antibody [NBP1-76544] - Glutaminase protein expression in PC3 and PC3M cell lines before/after CB-839 treatment. PC3 & PC3M cells were treated (+) or not treated (-) with GLS1 inhibitor CB-839 (1 uM) and their expression of GLS1 & GLS2 proteins determined by Western blot. Antibodies specific for GAC & KCA were utilized on two replicate samples, while GLS2 expression was determined on three. PC3 cell lysates expressed GLS2 more than PC3M cell lysates regardless of CB-839 treatment. The difference in GLS2 expression was significant in the untreated cells (P?<?0.03) but because of high variability the difference was not significant in the drug-treated cells. Levels of GLS1 (GAC and KGA) were similar in all cell lysates. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-017-16327-z), licensed under a CC-BY licence.
Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.
Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.
(5 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, Simple Western, WB
AGA Antibody
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Immunohistochemistry-Paraffin: AGA Antibody [NBP1-88866] - Staining of human epididymis shows high expression.
Immunohistochemistry-Paraffin: AGA Antibody [NBP1-88866] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: AGA Antibody [NBP1-88866] - Staining in human epididymis and skeletal muscle tissues using anti-AGA antibody. Corresponding AGA RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
RGL1 Antibody
(2D10)
H00023179-M01
Immunohistochemistry-Paraffin: RGL1 Antibody (2D10) [H00023179-M01] - Analysis of monoclonal antibody to RGL1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: RGL1 Antibody (2D10) [H00023179-M01] - Detection limit for recombinant GST tagged RGL1 is approximately 0.1ng/ml as a capture antibody.
Western Blot: RGL1 Antibody (2D10) [H00023179-M01] - Analysis of RGL1 expression in transfected 293T cell line by RGL1 monoclonal antibody (M01), clone 2D10.Lane 1: RGL1 transfected lysate(90.7 KDa).Lane 2: Non-transfected lysate.
(1 Publication)
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
ARHGAP9 Antibody
NBP2-15454
Western Blot: ARHGAP9 Antibody [NBP2-15454] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: ARHGAP9 Antibody [NBP2-15454] - SkHep1 xenograft, using ARHGAP9 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Species: Hu
Applications: IHC, IHC-P, WB
RLF Antibody (2G2)
H00006018-M05
Sandwich ELISA: RLF Antibody (2G2) [H00006018-M05] - Detection limit for recombinant GST tagged RLF is 0.3 ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Mu
Applications: ELISA, S-ELISA, WB
INSL3 Antibody
NBP1-81223
Immunohistochemistry-Paraffin: INSL3 Antibody [NBP1-81223] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: INSL3 Antibody [NBP1-81223] - Staining in human testis and endometrium tissues using anti-INSL3 antibody. Corresponding INSL3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: INSL3 Antibody [NBP1-81223] - Staining of human testis shows high expression.
(4 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
RalA/RalB Antibody
AF3204
Western blot shows lysates of U937 human histiocytic lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat RalA/RalB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3204) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human RalA and RalB (5 ng/lane) were included. A specific band for RalA/RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB
RASGRF1 Antibody
NBP1-85727
Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP1-85727] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: RASGRF1 Antibody [NBP1-85727] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & the Golgi apparatus. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
RGL3 Antibody
NBP1-92327
Western Blot: RGL3 Antibody [NBP1-92327] - Analysis in control (vector only transfected HEK293T lysate) and RGL3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: RGL3 Antibody [NBP1-92327] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: RGL3 Antibody [NBP1-92327] - Staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
Species: Hu
Applications: IHC, IHC-P, WB
SOS2 Antibody
[Unconjugated]
AF6260
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
SOS1 Antibody
(SOS-01) - BSA Free
NB110-68800
Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Species: Hu, Mu
Applications: ICC/IF, WB
Raf-1 Antibody
(563002)
MAB4540
Western blot shows lysates of MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Raf‑1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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(1 Publication)
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AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(26 Publications)
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SCFD1 Antibody
(OTI5G4)
NBP2-02031
Western Blot: SCFD1 Antibody (5G4) [NBP2-02031] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCFD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCFD1.
Immunohistochemistry-Paraffin: SCFD1 Antibody (5G4) [NBP2-02031] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SCFD1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SCFD1 Antibody (5G4) [NBP2-02031] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SCFD1 mouse monoclonal antibody.
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
TC21/R-Ras2
Antibody (2D3-4B8)
H00022800-M01
Western Blot: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Analysis of RRAS2 expression in transfected 293T cell line by RRAS2 monoclonal antibody (M01), clone 2D3-4B8.Lane 1: RRAS2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.
Knockdown Validated: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Identification of defatty-acylation targets of SIRT6 that contribute to its function in cell proliferation. Effects of R-Ras2 knockdown on the proliferation of Sirt6 WT and KO MEFs. Values with error bars indicate mean +- s.d. of three biological replicates. *** indicates p<0.005 for comparing to Sirt6 KO MEFs. Schematic overview of the SILAC experiment to compare the protein abundance in Sirt6 WT and KO. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/25158) licensed under a CC-BY licence.
ELISA: TC21/R-Ras2 Antibody (2D3-4B8) [H00022800-M01] - Detection limit for recombinant GST tagged RRAS2 is 1 ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
LIGHT/TNFSF14
[Unconjugated]
664-LI/CF
1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.
Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.
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RGL2 Antibody
NBP2-59128
RGL2 Antibody
Species: Hu
Applications: IP, WB (-)

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Bioinformatics

Gene Symbol RGL2