Retinol Binding Protein RBP Antibody

Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494] - Staining of NIH3T3 embryonic mouse cell lysate with antibody at 2 ug/mL (A) and U251 cell lysate with antibody at 0.5 ug/mL (B). 35 ug protein each in RIPA buffer.

Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed U-2 OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Retinol Binding Protein RBP Antibody Summary

• Immunogen: Peptide with sequence VEGVVCKQVFKKVQ corresponding to C-Terminus according to NP_002890.1.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.5 - 3 ug/mL
• Application Notes: IHC-P has been reported for previous lots.
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Retinol Binding Protein RBP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Retinol Binding Protein RBP Antibody

• C
• Cellular retinol-binding protein
• CRABP-I
• CRBP1CRBP-I
• CRBPCellular retinol-binding protein I
• CRBPI
• RBPC
• retinol binding protein 1, cellular
• retinol-binding protein 1
• retinol-binding protein 1, cellular

Background

RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Retinol Binding Protein RBP Antibody (NB100-1494)(1)

Publication using NB100-1494

• Farias EF, Ong DE, Ghyselinck NB et al. Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity. J Natl Cancer Inst 2005-01-05 [PMID: 15632377]

Bioinformatics

• Gene Symbol: RBP1
• Entrez:
  • 5947(Human)
  • 19659(Mouse)
• Uniprot:
  • P09455(Human)