Retinol Binding Protein RBP Antibody

Images

 
Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494] - staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NB100-1494] - staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data was obtained ...read more
Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NB100-1494] - (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Retinol Binding Protein RBP Antibody Summary

Immunogen
Peptide with sequence VEGVVCKQVFKKVQ corresponding to C-Terminus according to NP_002890.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 16 kDa band observed in Murine NIH 3T3 lysates (calculated MW of 15.9 kDa band according to NP_002890.1). IHC-P: Human pancreas shows vesicular staining in the acinar cytoplasm but oriented towards the interlobular space.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Retinol Binding Protein RBP Lysate (NBL1-15226)
Publications
Read Publication using NB100-1494.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Retinol Binding Protein RBP Antibody

  • C
  • Cellular retinol-binding protein
  • CRABP-I
  • CRBP1CRBP-I
  • CRBPCellular retinol-binding protein I
  • CRBPI
  • RBPC
  • retinol binding protein 1, cellular
  • retinol-binding protein 1
  • retinol-binding protein 1, cellular

Background

RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Retinol Binding Protein RBP Antibody (NB100-1494)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RBP1
Entrez
Uniprot