RENT1/UPF1/hUPF1 Antibody

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Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.
Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RENT1/UPF1/hUPF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1075 and the C-terminus (residue 1118) of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UPF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2500 (ECL)
  • Immunoprecipitation 2 -10 ug/mg lysate
Application Notes
By Western blot, this antibody recognizes a band at ~145 kDa, representing human RENT1. *The investigator should determine the optimal working dilution for a specific application.
Publications
Read Publication using NB100-370.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RENT1/UPF1/hUPF1 Antibody

  • ATP-dependent helicase RENT1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ43809
  • HUPF1
  • KIAA0221FLJ46894
  • Nonsense mRNA reducing factor 1
  • NORF1
  • NORF1delta helicase
  • pNORF1
  • regulator of nonsense transcripts 1
  • RENT1
  • RENT1UP Frameshift 1
  • UPF1 regulator of nonsense transcripts homolog (yeast)
  • UPF1
  • up-frameshift mutation 1 homolog
  • Up-frameshift suppressor 1 homolog
  • yeast Upf1p homolog

Background

RENT1 (regulator of nonsense transcripts 1) is a group 1 RNA helicase, RNA-binding protein whose RNA-binding activity is modulated by ATP. It enters the nucleus where it influences early events in mRNA biogenesis. It functions in NMD (non-sense-mediated mRNA decay) in mammalian cells, ridding cells of aberrant mRNAs containing premature termination codons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RENT1/UPF1/hUPF1 Antibody (NB100-370)(1)

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Bioinformatics

Gene Symbol UPF1
Entrez
Uniprot