Reg1B Overexpression Lysate (Native) (NBL1-15272)

Images

 
Western Blot: REG1B Overexpression Lysate (Adult Normal) [NBL1-15272] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for REG1B.

Product Details

Summary
Applications WB

Order Details

Reg1B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206451 and based on accession number NM_006507. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens regenerating islet-derived 1 beta (REG1B), mRNA.
Gene
REG1B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Reg1B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Reg1B Overexpression Lysate (Native)

  • Lithostathine 1 beta
  • lithostathine-1-beta
  • Pancreatic stone protein 2
  • pancreatic threadprotein)
  • PSPS2
  • PSPS2PSP-2
  • Reg1B
  • regenerating islet-derived 1 beta
  • Regenerating islet-derived protein 1-beta
  • Regenerating protein I beta
  • REGH
  • REGLREG-1-beta
  • secretory pancreatic stone protein 2

Background

This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins. This gene encodes a protein secreted by the exocrine pancreas that is highly similar to the REG1A protein. The related REG1A protein is associated with islet cell regeneration and diabetogenesis, and may be involved in pancreatic lithogenesis. Reg family members REG1A, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Reg1B Lysate (NBL1-15272) (0)

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Bioinformatics

Gene Symbol REG1B
Entrez

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