Human Reg1B ELISA Kit (Colorimetric)

ELISA: Human Reg1B ELISA Kit (Colorimetric) [NBP2-82147] - Samples were spiked with high concentrations of Human Reg1B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Reg1B ELISA Kit (Colorimetric) [NBP2-82147] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.31 - 20ng/mL
• Sensitivity: 0.19 ng/mL

Human Reg1B ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Reg1B in samples. No Significant cross-reactivity or interference between Human Reg1B and analogues was observed.
• Standard Curve Range: 0.31 - 20ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 6%
• Intra-Assay: CV% < 5%
• Spike Recovery: average = 94% for serum; average = 95% for EDTA plasma; average = 95% for cell culture media
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: REG1B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Reg1B ELISA Kit (Colorimetric)

• Lithostathine 1 beta
• lithostathine-1-beta
• Pancreatic stone protein 2
• pancreatic threadprotein)
• PSPS2
• PSPS2PSP-2
• Reg1B
• regenerating islet-derived 1 beta
• Regenerating islet-derived protein 1-beta
• Regenerating protein I beta
• REGH
• REGLREG-1-beta
• secretory pancreatic stone protein 2

Background

REG1B is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins. This gene encodes a protein secreted by the exocrine pancreas that is highly similar to the REG1A protein. The related REG1A protein is associated with islet cell regeneration and diabetogenesis, and may be involved in pancreatic lithogenesis. Reg family members REG1A, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Reg1B ELISA Kit (NBP2-82147). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: REG1B