Recombinant Mouse Wnt-8a Protein, CF

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Recombinant Mouse Wnt-8a stimulates alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL.
1 μg/lane ofRecombinant Mouse Wnt-8a (Catalog # 8419-WN/CF)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing bands at 42.3 and 38.9 kDa, ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Wnt-8a Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-8a protein
Ala20-Trp354
Accession #
N-terminal Sequence
Ala20 & Trp23
Protein/Peptide Type
Recombinant Proteins
Gene
Wnt8a
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-62 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-8a Protein, CF

  • protein Wnt-8a
  • Protein Wnt-8d
  • wingless-type MMTV integration site family, member 8A
  • Wnt8a
  • Wnt-8a
  • WNT8DWNT8d

Background

Wnt-8a is a member of the large and highly conserved Wnt family of signaling molecules that have roles in pattern formation, cell fate decision, axon guidance, and tumor formation (1). Mouse Wnt-8a consists of a 19 amino acid (aa) signal peptide that is cleaved to release the mature 335 aa secreted protein (2). Mature mouse Wnt-8a shares 82% and 91% sequence identity with human and rat mature Wnt-8a, respectively. Similar to other canonical Wnts, Wnt-8a binds to the Frizzled family of receptors to intiate beta-catenin signaling. Wnt-8a is expressed during early embryogenesis and is involved in mesoderm patterning and posteriorization of the neuroectoderm (3-6). Wnt-8a signaling promotes optic lens development as well as otic placode formation during inner ear development (7, 8). Expression of Wnt-8a is observed in heart progenitor cells where it positively regulates cardiac myogenesis (9, 10).

  1. Anastas, J.N. and R.T. Moon (2013) Nat. Rev. Cancer 13:11.
  2. Bouillet, P. et al. (1996) Mech. Dev. 58:141.
  3. Rhinn, M. et al. (2005) Development 132:1261.
  4. Lekven, A.C. et al. (2001) Dev. Cell. 1:103.
  5. Erter, C.E. et al. (2001) Development 128:3571.
  6. Stump, R.J. et al. (2003) Dev. Biol. 259:48.
  7. Vendrell, V. et al. (2013) Mech. Dev. 130:160.
  8. Chong, C.C. et al. (2009) Exp. Eye Res. 88:307.
  9. Nakamura, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5834.
  10. Kwon, C. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10894.

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Bioinformatics

Gene Symbol Wnt8a
Entrez
Uniprot