Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse TGF- beta 2 (Catalog # 7346-B2) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse Vasorin/SLIT-like 2 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL
• Source: Mouse myeloma cell line, NS0-derived mouse Vasorin/SLIT-like 2 protein
  Cys25-Pro576, with a C-terminal 6-His tag
• Accession #: Q9CZT5
• N-terminal Sequence: Cys25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Vasn
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 72-97 kDa, reducing conditions

Packaging, Storage & Formulations

• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Ethylene Glycol and Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

• Atia
• Protein slit-like 2
• SLIT like 2
• Slitl2
• slit-like 2 (Drosophila)
• slit-like 2
• VASN
• Vasorin

Background

Vasorin, also known as SLIT-like 2, is an approximately 110 kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95 kDa soluble form of Vasorin can be shed from the cell surface by ADAM17 mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature mouse Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type-III domain, a 21 aa transmembrane segment, and a 76 aa cytoplasmic domain (1). Within the ECD, mouse Vasorin shares 85% and 96% aa sequence identity with human and rat Vasorin, respectively.

1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
2. Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
3. Malapeira, J. et al. (2011) Oncogene 30:1912.
4. Li, S. et al. (2015) Oncotarget 6:10045.

Bioinformatics

• Gene Symbol: Vasn
• Uniprot:
  • Q9CZT5()