Recombinant Mouse SNED1 Protein, CF

Immobilized Recombinant Mouse SNED1 (Catalog #9335-SN) supports the adhesion of CCD-1070Sk human normal skinfibroblasts The ED50 for this effect is0.1-0.4 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SNED1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of CCD-1070Sk human normal skin fibroblasts. The ED₅₀ for this effect is 0.1-0.4 μg/mL
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse SNED1 protein
  Arg23-Lys1403, with a C-terminal 6-His tag
• Accession #: Q70E20
• N-terminal Sequence: Leu28
• Protein/Peptide Type: Recombinant Proteins
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 135-230 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SNED1 Protein, CF

• DKFZp586B2420
• FLJ00133,6720455I24Rik homolog
• insulin responsive sequence DNA binding protein-1
• Insulin-responsive sequence DNA-binding protein 1
• IRE-BP1
• SNED1
• Snep
• SST3
• sushi, nidogen and EGF-like domain-containing protein 1
• sushi, nidogen and EGF-like domains 1

Background

SNED1 (Sushi, nidogen and EGF-like domain-containing protein 1), also known as stromal nidogen extracellular matrix protein (Snep) and secreted protein SST-3, is an approximately 150 kDa protein (1). Mature mouse SNED1 contains 1379 aa that includes a Sushi/CCP (complement control protein) domain, an NIDO domain, 15 EGF-like domains, and 3 fibronectin type-III domains (1). Mouse SNED1 shares 84% and 96% aa sequence identity with human and rat SNED1, respectively. Alternative splicing generates an isoform lacking the Sushi/CCP domain, normally found in the center of the protein. SNED1 is mainly expressed in kidney stromal cells, but can be found in mesenchymal cells of other embryonic tissues, and within the nervous system (1,2). SNED1 has been found to have a role in tumor progression and metastasis. Studies show that SNED1 is up-regulated in highly metastatic mammary tumors while it is found to have relatively lower expression levels in poorly metastatic mammary tumors (3). SNED1 has also been shown to be up-regulated in pancreatic ductal adenocarcinoma cells which correlates with an increased chemoresistance (4). Additionally, SNED1 is found to be expressed specifically in stroma cells of the kidney and other organs in areas that undergo apoptosis during embryonic development making it important for kidney development (1).

1. Leimeister, C. et al. (2004) Developmental Dynamics. 230:371.
2. Ueno, H. et al. (2003) Nature Immunology. 4:457.
3. Naba, A. et al. (2014) eLife. 3:e01308.
4. Longati, P. et al. (2013) BMC Cancer. 13:95.

Publications for SNED1 (9335-SN)(2)

Publications using 9335-SN

• Pally, D;Kapoor, N;Naba, A; The novel ECM protein SNED1 mediates cell adhesion via the RGD-binding integrins ?5?1 and ?v?3 Journal of cell science 2024-12-23 [PMID: 39713860] (Bioassay, Mouse)
• Pally, D;Kapoor, N;Naba, A; The novel ECM protein SNED1 mediates cell adhesion via ?5?1 integrin bioRxiv : the preprint server for biology 2024-08-08 [PMID: 39149327] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • Q70E20()