Recombinant Mouse SMOC-2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SMOC-2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.

  When 5 x 10⁴ cells/well are added to rmSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED₅₀ for this effect is 0.2‑0.8 μg/mL.

• Source: Mouse myeloma cell line, NS0-derived mouse SMOC-2 protein
  Leu30-Gly447, with a C-terminal 6-His tag
• Accession #: AAH19527
• N-terminal Sequence: Leu30
• Protein/Peptide Type: Recombinant Proteins
• Gene: Smoc2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 58-61 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SMOC-2 Protein, CF

• bA270C4A.1
• bA37D8.1
• dJ421D16.1
• MST117
• MSTP117
• MSTP140
• SMAP2
• SMAP-2
• SMAP2Secreted modular calcium-binding protein 2
• SMOC2
• SMOC-2
• smooth muscle associated protein 2
• Smooth muscle-associated protein 2
• SPARC related modular calcium binding 2
• SPARC-related modular calcium-binding protein 2
• thyroglobulin type-1 repeat containing protein

Background

SMOC-2 (secreted, or SPARC-related, modular calcium-binding protein 2), previously called SMAP2 (smooth muscle-associated protein 2), is a 55 kDa glycoprotein that is a member of the SPARC family of matricellular proteins (1, 2). The mouse SMOC-2 cDNA encodes 447 amino acids (aa), including a 21 aa signal sequence, a Kazal-like domain (aa 40 - 84), two thyroglobulin type-1 segments (aa 87 - 153 and 213 - 281) and two EF-hand sequences (aa 347 - 382 and 384 - 419). Of three splice variants, one shows a 13 aa substitution for aa 443 - 447, another shows an 11 aa insertion after Ala170, and a third contains both of these variations. Mature mouse SMOC-2 shares 99%, 95% and 93% aa identity with rat, human and canine SMOC-2, respectively. SMOC-2 is widely expressed in the extracellular matrix and appears to have adhesion-related functions (1 - 5). Recombinant bacterially produced human SMOC-1 and SMOC-2 can bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin, while keratinocyte SMOC-2 binds integrins alpha v beta 1 and alpha v beta 6 (3, 6). SMOC-2 promotes cell cycle progression by signaling through the integrin-linked kinase (ILK) to upregulate cyclin-D1 (4). When expressed in the endothelial extracellular matrix, it potentiates growth factor-induced angiogenesis (5). SMOC-2 expression is upregulated during neointima formation, promoting proliferation and migration of vascular smooth muscle (2). In the skin, it promotes keratinocyte attachment and migration (6). Since Kazal and thyroglobulin domains are often found in protease inhibitors, SMOC-2 is also proposed to inhibit proteases in the lung and artery (2, 7).

1. Vannahme, C. et al. (2003) Biochem. J. 373:805.
2. Nishimoto, S. et al. (2002) Biochim. Biophys. Acta 1576:225.
3. Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
4. Liu, P. et al. (2008) Mol. Biol. Cell 19:248.
5. Rocnik, E.F. et al. (2006) J. Biol. Chem. 281:22855.
6. Maier, S. et al. (2008) Exp. Cell Res. 314:2477.
7. Wilk, J.B. et al. (2007) Am. J. Respir. Crit. Care Med. 175:554.

Publications for SMOC-2 (6075-SM)(1)

Publication using 6075-SM

• SC Schüler, JM Kirkpatric, M Schmidt, D Santinha, P Koch, S Di Sanzo, E Cirri, M Hemberg, A Ori, J von Maltza Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality Cell Reports, 2021-06-08;35(10):109223. 2021-06-08 [PMID: 34107247] (Bioassay, In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Smoc2
• Uniprot:
  • AAH19527()