Recombinant Mouse SMOC-1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SMOC-1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.

  When 5 x 10 4 cells/well are added to rmSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 degreesC. The ED 50 for this effect is 1-4 μg/mL.

• Source: Mouse myeloma cell line, NS0-derived mouse SMOC-1 protein His26-Val452, with a C-terminal 6-His tag
• Accession #: AAD41590
• N-terminal Sequence: His26 & Ile35
• Protein/Peptide Type: Recombinant Proteins
• Gene: Smoc1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SMOC-1 Protein, CF

• Secreted modular calcium-binding protein 1
• SMOC1
• SMOC-1
• SPARC related modular calcium binding 1
• SPARC-related modular calcium-binding protein 1

Background

SMOC-1 (secreted, or SPARC-related, modular calcium-binding protein 1) is a 70 - 90 kDa secreted glycoprotein that is a member of the SPARC family of matricellular molecules (1). Mature mouse SMOC-1 is 427 amino acids (aa) in length. It contains one Kazal-like domain (aa 42 - 86), two thyroglobulin type-1 segments (aa 91 - 157 and223 - 291) and two functional EF-hand sequences (aa 358 - 393 and 395 - 430) (1, 2). Two splice variants contain an insertion of eleven aa after Lys174, with one of these also including an alternate start site at Met65 (3). Mature mouse SMOC-1 shares 99% aa identity with rat SMOC-1 and 92% aa identity with human, canine and bovine SMOC-1. The principal difference between rodents and other mammals is an additional 19 aa near the C-terminus of rodent SMOC-1. Like other matricellular proteins, SMOC-1 is primarily expressed in basement membranes, although it has also been found in other extracellular matrices and the oocyte zona pellucida (1). It is present early in mouse embryogenesis, and is produced by cells deriving from all three germ layers (4). Recombinant bacterially produced human SMOC-1 and SMOC-2 were both shown to bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin (2). A signaling role for SMOC-1 was shown in rat mesangial cells: induction of nitric oxide in response to inflammatory cytokines downregulates SMOC-1 which, in turn, downregulates expression of TGF-beta and TGF-beta -regulated genes. This mechanism is proposed to limit the profibrotic effects of TGF-beta , for example in the glomerulus (5). In Xenopus, the SMOC paralog has been shown to antagonize BMP activity.

1. Vannahme, C. et al. (2002) J. Biol. Chem. 277:37977.
2. Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
3. Entrez Q8BLY1 and EDL02699.
4. Gersdorff, N. et al. (2006) Histochem. Cell Biol. 126:705.
5. Dreieicher, E. et al. (2009) J. Am. Soc. Nephrol. 20:1963.
6. Thomas, J.T. et al. (2009) J. Biol. Chem. 284:18994.

Publications for SMOC-1 (5550-SM)(1)

Publication using 5550-SM

• Zhao, ZY;Siow, Y;Liu, LY;Li, X;Wang, HL;Lei, ZM; The SPARC-related modular calcium binding 1 (Smoc1) regulated by androgen is required for mouse gubernaculum development and testicular descent Asian journal of andrology 2024-08-09 [PMID: 39119686] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Smoc1
• Uniprot:
  • AAD41590()