Recombinant Mouse sFRP-3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse sFRP-3 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit proliferation in MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 1.5‑6.0 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse sFRP-3 protein
  Ala33-Ser323 with a C-terminal 6-His tag
• Accession #: P97401
• N-terminal Sequence: Ala33
• Protein/Peptide Type: Recombinant Proteins
• Gene: Frzb
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse sFRP-3 Protein, CF

• FIZ
• FREOS1
• Frezzled
• Fritz
• Frizzled-related protein 1
• frizzled-related protein
• FRP
• FRP-3
• FRZB
• Frzb1
• FRZB-1
• FRZB1sFRP-3
• FRZB-PEN
• FZRB
• hFIZ
• secreted frizzled-related protein 3
• sFRP3
• sFRP-3
• SFRP3frezzled
• SRFP3frizzled homolog-related

Background

Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. sFRP-3 is often referred to as FRZB, other names also include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The mouse protein shares 76% identity with Xenopus, and 92% with human proteins. The gene for mouse sFRP-3 has been localized to the central region of chromosome 2. Murine sFRP-3 is expressed in the primitive streak during gastrulation, as well as in the retina, foregut diverticulum, nervous system, and posterior mesoderm during development. In adult tissues, sFRP-3 expression, as determined by Northern blot, is detected in the heart, brain, spleen, skeletal muscle, kidney and testis.

The N-terminal portion of sFRP-3 protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. sFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.

1. Hoang et al. (1996) J. Biol. Chem. 271:26131.
2. Leyns et al. (1997) Cell 88:747.
3. Wang et al. (1997) Cell 88:757.
4. Mayr et al. (1997) Mech. Dev. 63:109.
5. Rattner et al. (1997) PNAS 94:2859.

Publications for sFRP-3/FRZB (592-FR/CF)(6)

Publications using 592-FR/CF

• C Chen, Y Yang, Y Yao HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/beta-Catenin and BMP2 Signaling Pathways Cell Transplant, 2019-11-06;0(0):9636897198835. 2019-11-06 [PMID: 31694396] (Rat)
• Li B, Shi Y, Shu J, Gao J, Wu P, Tang S Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways. J Biol Chem, 2013-03-28;288(19):13610-9. 2013-03-28 [PMID: 23539626] (In Vivo, Mouse)
• Jang M, Bonaguidi M, Kitabatake Y, Sun J, Song J, Kang E, Jun H, Zhong C, Su Y, Guo J, Wang M, Sailor K, Kim J, Gao Y, Christian K, Ming G, Song H Secreted frizzled-related protein 3 regulates activity-dependent adult hippocampal neurogenesis. Cell Stem Cell, 2013-02-07;12(2):215-23. 2013-02-07 [PMID: 23395446] (In Vivo, Mouse)
• Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L Wnt3a binds to several sFRPs in the nanomolar range. Biochem. Biophys. Res. Commun., 2007-04-19;357(4):1119-23. 2007-04-19 [PMID: 17462603] (Bioassay, Surface Plasmon Resonance, Mouse)
• Person AD, Garriock RJ, Krieg PA, Runyan RB, Klewer SE Frzb modulates Wnt-9a-mediated beta-catenin signaling during avian atrioventricular cardiac cushion development. Dev. Biol., 2005-02-01;278(1):35-48. 2005-02-01 [PMID: 15649459] (Bioassay, Chicken)
• Polesskaya A, Seale P, Rudnicki MA Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration. Cell, 2003-06-27;113(7):841-52. 2003-06-27 [PMID: 12837243] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Frzb
• Uniprot:
  • P97401()