Recombinant Mouse RELT/TNFRSF19L Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse RELT/TNFRSF19L Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED₅₀ for this effect is 0.3-1.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse RELT/TNFRSF19L protein
  

  Mouse RELT/TNFRSF19L (Met1-Gln169) Accession # Q8BX43	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q8BX43
• N-terminal Sequence: Thr32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Relt
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse RELT/TNFRSF19L Fc Chimera Protein, CF

• Receptor expressed in lymphoid tissues
• RELT tumor necrosis factor receptor
• RELT
• TNFRSF19L
• TNFRSF19LFLJ14993
• tumor necrosis factor receptor superfamily member 19L
• tumor necrosis factor receptor superfamily, member 19-like

Background

RELT (Receptor Expressed in Lymphoid Tissues) is a 46 kDa (predicted) type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily and designated TNFRSF19‑like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes (2, 3). Mouse RELT cDNA encodes 436 amino acids (aa), including a 31 aa signal peptide, a 138 aa extracellular domain (ECD) containing a TNF receptor cysteine‑rich domain and a potential N‑linked glycosylation site, a 21 aa transmembrane domain, and a 246 aa cytoplasmic region that lacks a death domain (2). Within the ECD, mouse RELT shares 78%, 89%, 70%, 70% and 67% aa sequence homology with human, rat, canine, porcine and bovine RELT, respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TROY/TNFRSF19 and OX40 (2). Neither human nor mouse RELT bind any of the ~19 TNF superfamily ligands that have been tested (1). Two related transmembrane proteins, RELL1 and RELL2, have been identified in both human and mouse (3). RELL1 and 2 are coexpressed with and can interact with RELT, and are thought to modulate its signaling (3‑5). Intracellularly, RELT has been shown to bind the adaptor protein TRAF‑1 and activate the NF‑ kappa B pathway, the phospholipid scramblase PLSCR1, and the oxidative stress responsive protein OSR1 (2‑5). Another investigator notes association and signaling through SPAK, but not TRAF or NF kappa B (6). When overexpressed in HEK‑293 cells, RELT induces p38 and JNK signaling and activates apoptosis (4‑6).

1. Bossen C. et al. (2006) J. Biol. Chem. 281:13964.
2. Sica, G. et al. (2001) Blood 97:2702.
3. Cusick, J.K. et al. (2006) Biochem. Biophys. Res. Commun. 340:535.
4. Cusick, J.K. et al. (2010) Cell. Immunol. 261:1.
5. Cusick, J.K. et al. (2012) Mol. Cell. Biochem. 362:55.
6. Polek T.C. et al. (2006) Biochem. Biophys. Res. Commun. 343:125.

Bioinformatics

• Gene Symbol: Relt
• Uniprot:
  • Q8BX43()