Recombinant Mouse Plexin A1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Plexin A1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When recombinant mouse Plexin A1 is coated at 2.5 μg/mL (100 μL/well), recombinant human Semaphorin 6C/Fc Chimera binds with an apparent K_D <5 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Plexin A1 protein
  Ser28-Pro1242, with a C-terminal 6-His tag
• Accession #: P70206
• N-terminal Sequence: Ser28
• Protein/Peptide Type: Recombinant Proteins
• Gene: Plxna1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 134.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 150-160 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Plexin A1 Protein, CF

• NOV
• NOVP
• Plexin A1
• PLEXIN-A1
• PLXN1
• PLXNA1

Background

Plexin A1 (formerly Plexin 1) is a 200 kDa type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). Plexin signaling induces cytoskeletal remodeling, which mediates cell migration and axon repulsion (2). The mouse Plexin A1 cDNA encodes 1894 amino acids (aa) including a 27 aa signal sequence, a 1215 aa extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain (1). Within the ECD, human Plexin A1 shares 95%, 95%, 92%, 80% and 79% aa sequence identity with mouse, rat, bovine, chicken and Xenopus Plexin A1, respectively. The four mouse Plexin A molecules share 59 ‑ 67% aa identity with each other. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2, and binds transmembrane Semaphorin 6D directly (3 ‑ 5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5 ‑ 8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12).

1. Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
2. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
3. Takamatsu, H. et al. (2010) Cell. Mol. Immunol. 7:83.
4. O’Connor, B.P. and J.P.Y. Ting (2008) Immunol. Res. 41:217.
5. Takahashi, T. et al. (1999) Cell 99:59.
6. Yoshida, Y. et al. (2006) Neuron 52:775.
7. Toyofuku, T. et al. (2005) Nat. Neurosci. 8:1712.
8. Nawabi, H. et al. (2010) Genes Dev. 24:396.
9. Eun, S-Y. et al. (2006) J. Immunol. 177:4271.
10. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
11. Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
12. Catalano, A. et al. (2009) Cancer Res. 69:1485.

Bioinformatics

• Gene Symbol: Plxna1
• Uniprot:
  • P70206()