Recombinant Mouse NgR3/NgRH2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse NgR3/NgRH2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human APP695 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse NgR3/NgRH2 that produces 50% optimal binding response is 0.03-0.18 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse NgR3/NgRH2 protein
  Cys25-Ala423, with a C-terminal 6-His tag
• Accession #: Q8K0S5
• N-terminal Sequence: Cys25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Rtn4rl1
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 61-69 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse NgR3/NgRH2 Protein, CF

• DKFZp547J144
• NgR3
• NgRH2
• NGRL2
• nogo receptor-like 2
• Nogo-66 receptor homolog 2
• Nogo-66 receptor-related protein 3
• reticulon 4 receptor-like 1
• reticulon-4 receptor-like 1
• RTN4RL1

Background

NgR3/NgRH2 (also known as reticulon-4 receptor-Like 1), along with the related NgR1 and NgR2, is a member of the Nogo66 receptor (NgR) family. NgR proteins are expressed primarily by neurons in the central and peripheral mammalian nervous system. NgR proteins play an essential role in mediating axon growth inhibition induced by the structurally distinct proteins Nogo, myelin associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMgp) (1, 2).These proteins belong to the family of myelin-associated inhibitors (MAIs). NgR proteins are composed of 8 leucine-rich repeats (LRRs) flanked by N-terminal and C-terminal leucine-rich capping domains and are attached at the cell surface by a C-terminal glycosylphosphatidylinositol (GPI) anchor (3). Because NgR proteins have no transmembrane domain, they must be associated with membrane proteins in order to transduce cell signaling (2).  Both NGF R/TNFRSF16 (p75) and TROY/TNFRSF19 have been identified as NgR1-associated components (4, 5). Mouse NgR3 shares 88% and 96% amino acid sequence identity with human and rat NgR3 respectively. NgR1 and NgR3 serve as receptors for chondroitin sulfate proteoglycans (CSPGs) (6). CSPGs and MAIs are known to stabilize neuronal structure and influence the growth and guidance of developing neurons (7). Therefore, NgR proteins are part of a multicomponent receptor system implicated in pathways that limit neuronal growth and increase the structural stability of synapses.

1. GrandPre, T. et al. (2002) Nature 417:547.
2. Wang, K.C. et al. (2002) Nature 420:74.
3. Fournier, A.E. et al. (2001) Nature 409:341.
4. Shao, Z. et al. (2005) Neuron 45:353.
5. Park, J. B. et al. (2005) Neuron 45:345.
6. Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.
7. Kantor, D.B. et al. (2004) Neuron 44:961.

Bioinformatics

• Gene Symbol: Rtn4rl1
• Uniprot:
  • Q8K0S5()