Recombinant Mouse Nectin-4 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Nectin-4 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. When 5 x 10⁴ cells/well are added to recombinant mouse Nectin-4 coated plates (10 µg/mL with 100 µL/well), approximately 45%-65% will adhere after 1 hour incubation at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse Nectin-4 protein
  Tyr28-Ile349, with a C-terminal 6-His tag
• Accession #: NP_082169
• N-terminal Sequence: Tyr28
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Nectin4
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-50 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Nectin-4 Protein, CF

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins (1). The Latin word necto means “to connect”, indicating the role of nectins in Ca^2+‑independent cell-cell adhesion (2). Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4 (1 ‑ 3). Mouse Nectin-4 mRNA is found in the embryo, lung, testis and brain, while in the human it is normally expressed in the placenta, especially in endothelial cells (1, 4, 5). Mouse Nectin-4 cDNA encodes 508 amino acids (aa), including a 30 aa signal sequence, a 317 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type (3). One isoform lacks aa 410-434 in the cytoplasmic domain (1). In many human ductal breast or non-small cell lung carcinomas, Nectin-4 is up-regulated and a soluble 43 kDa form is found in the plasma (4 ‑ 6). This form is generated from the membrane protein via the action of TACE/ADAM-17 (6). The extracellular domain of mouse Nectin-4 shares 91%, 97%, 92%, 90% and 89% amino acid sequence homology with the corresponding regions of human, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins (1, 2). These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections (2, 7).

1. Reymond, N. et al. (2001) J. Biol. Chem. 276:43205.
2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
4. Fabre-Lafay, S. et al. (2007) BMC Cancer 7:73.
5. Takano, A. et al. (2009) Cancer Res. 69:6694.
6. Fabre-Lafay, S. et al. (2005) J. Biol. Chem. 280:19543.
7. Brancati, F. et al. (2010) Am. J. Hum. Genet. 87:265.

Bioinformatics

• Gene Symbol: Nectin4
• Uniprot:
  • NP_082169()