Recombinant Mouse Mill2 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Mill2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of EL‑4 mouse lymphoblast cells. The ED₅₀ for this effect is 1-5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Mill2 protein
  

  Mouse Mill2 (Ser30-His298) Accession # NP_715641	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: NP_715641
• N-terminal Sequence: Ser30
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Mill2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-66 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Mill2 Fc Chimera Protein, CF

• Mill2

Background

Mill2 (MHC class I‑like located near the leukocyte receptor complex‑2) is an ~41 kDa glycosyl‑phosphatidylinositol (GPI)‑linked glycoprotein that is a member of the non‑classical MHC class I‑like family (1‑3). Mouse Mill2 cDNA encodes 340 amino acids (aa) that include a signal sequence, an MHC I domain containing alpha 1, alpha 2 and alpha 3 regions, and a GPI anchor sequence (1). A long form contains 15 aa inserted near the N‑terminus. Mature mouse Mill2 (short form) shares 74% aa sequence identity with rat Mill2 and approximately 61% aa identity with mouse Mill1. Human orthologs of Mill proteins have not been identified, although similarities in structure and sequence with human HFE and MICA/B have been noted (1, 4, 5). Mill2 is expressed on proliferating thymocytes, smooth muscle cells and fibroblasts in skin, bladder, uterus and colonic muscularis (5). Low expression is detected in many tumor cell lines (5). Mill proteins bind beta 2‑microglobulin (2). While Mill1 requires beta 2‑microglobulin for surface expression, the short form of Mill2 does not, and surface expression of the long form is higher in its presence (5). Mill proteins are unlikely to present peptides and do not bind to NKG2D (2, 5). Recombinant Mill2 has, however, shown pH‑dependent binding to TfR/CD71‑expressing immature cells in the B lymphocyte and erythrocyte lineages, as well as activated, proliferating splenic T and B cells and some cell lines (5).

1. Kasahara, M. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13687.
2. Kajikawa, M. et al. (2006) J. Immunol. 177:3108.
3. Martinez-Naves, E. et al. (2011) FEBS Lett. 585:3478.
4. Watanabe, Y. et al. (2004) Eur. J. Immunol. 34:1597.
5. Rabinovich, B.A. et al. (2008) Immunol. Cell Biol. 86:489.

Bioinformatics

• Gene Symbol: Mill2
• Uniprot:
  • NP_715641(Mouse)