Recombinant Mouse Meteorin-like/METRNL Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Meteorin-like/METRNL Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. This protein is able to significantly enhance neurite outgrowth in the range of 60 ng/3 μL drop‑300 ng/3 μL drop.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Meteorin-like/METRNL protein
  Gln46-Glu311
• Accession #: Q8VE43
• N-terminal Sequence: No results obtained: Gln46 predicted
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Metrnl
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 29.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 33-37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Meteorin-like/METRNL Protein, CF

• Meteorin like
• meteorin, glial cell differentiation regulator-like
• meteorin-like protein
• Meteorin-like
• METRNL
• MGC99788

Background

Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1 ‑ 3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2 ‑ 5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).

1. Park, J.A. et al. (2008) Glia 56:247.
2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
4. Nishino, J. et al. (2004) EMBO J. 23:1998.
5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
6. Accession # Q8VE43.
7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.
8. Surace, C. et al. (2009) Clin. Genet. 76:256.

Publications for Meteorin-like/METRNL (6679-MN)(3)

Publications using 6679-MN

• K Delaunay, A Sellam, V Dinet, A Moulin, M Zhao, E Gelizé, J Canonica, MC Naud, P Crisanti-L, F Behar-Cohe Meteorin Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration Journal of Clinical Medicine, 2021-07-02;10(13):. 2021-07-02 [PMID: 34279457] (In Vivo, Rats)
• Li Z, Song J, Zheng S, Fan M, Guan Y, Qu Y, Xu J, Wang P, Miao C Adipocyte Metrnl Antagonizes Insulin Resistance Through PPARgamma Signaling. Diabetes, 2015-08-25;64(12):4011-22. 2015-08-25 [PMID: 26307585] (Bioassay, Hamster)

Bioinformatics

• Gene Symbol: Metrnl
• Uniprot:
  • Q8VE43()