>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
3 kDa & 26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
27 kDa (major) and minor auto-activation fragments, reducing conditions
Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
Dilute rmMatriptase to 0.2 ng/µL in Assay Buffer.
Dilute Substrate to 50 µM in Assay Buffer.
Load into a plate 50 µL of 0.2 ng/µL rmMatriptase, and start the reaction by adding 50 µL of 50 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 50 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
Calculate specific activity:
Specific Activity (pmol/min/µg) =
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)
*Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (Sigma, Catalog # A9891).
rmMatriptase: 0.010 µg
Substrate: 25 µM
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF
Membrane-type serine protease 1
Serine protease 14
Serine protease TADG-15
suppression of tumorigenicity 14 (colon carcinoma)
suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
suppressor of tumorigenicity 14 protein
tumor associated differentially expressed gene 15 protein
Tumor-associated differentially-expressed gene 15 protein
Matriptase, a mouse type II membrane serine protease encoded by the ST14 (suppression of tumorigenicity 14) gene, is also known as epithin, and membrane-type serine protease 1/MT-SP1 (1-2). Its human ortholog MT-SP1/Matriptase (Catalog # 3946-SE), which shares 81% amino acid identity with epithin, has been thought to play an important role in tumor biology and is a potential target for anti-cancer therapy (3). Matriptase has a multidomain structure containing a putative N-terminal transmembrane region, two CUB domains, four LDLRA repeats, and a C-terminal serine protease domain (1). The protease domain of epithin starts with Val615. R&D Systems recombinant mouse Matriptase is an active protease and consists of the catalytic domain (Val615 to Val855) and a short peptide (Gly596 to Arg614).
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