Recombinant Mouse IL-28A/IFN-lambda 2 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-28A/IFN-lambda 2 Protein Summary

Details of Functionality
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 0.75-3.75 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-28A/IFN-lambda 2 protein
Asp20-Val193, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asp20
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
25 kDa and 30 kDa, reducing conditions
Publications
Read Publications using
4635-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-28A/IFN-lambda 2 Protein

  • interleukin-28A
  • IFN-lambda 2
  • IL28A
  • IL-28A
  • interferon, lambda 2

Background

IL-28A (also named interferon-lambda 2, IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1 - 4). They are distantly related to members of the IL-10 family and type I IFN family (1 - 4). Mouse IL-28A cDNA encodes a 193 amino acid (aa) protein with a 19 aa signal peptide and a 174 aa mature protein that lacks N-glycosylation sites. Mature mouse IL-28A shares 81% and 66% aa sequence identity with rat and human IL-28A, respectively, and functions across species (5). Mouse IL-28A and IL-28B share 97% aa identity; the mouse lacks a functional IL-29 gene (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2 - 6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor  beta (IL-10 R beta ) and IL-28 receptor  alpha (IL-28 R alpha ; also called IFN-lambda  R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1 - 3). Both type I and III IFNs induce antiviral activity and upregulate MHC class I antigen expression (2 - 6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust antiviral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9 - 11).

  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
  5. Ank, N. et al. (2006) J. Virol. 80:4501.
  6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
  7. Siebler, J. et al. (2007) Gastroenterology 132:358.
  8. Meager, A. et al. (2005) Cytokine 31:109.
  9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  10. Sato, A. et al. (2006) J. Immunol. 176:7686.
  11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.

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4635-ML
Species: Mu
Applications: Bioactivity

Publications for IL-28A/IFN-lambda 2 (4635-ML)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


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Bioassay
(2)
Cell Culture
(1)
In Vivo
(1)
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