Recombinant Mouse IL-20 R beta Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse IL-20 R beta Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind recombinant mouse IL-20 in a functional ELISA with an estimated K_D<10 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse IL-20 R beta protein
  

  Mouse IL-20 R beta (Asp25-Gly223) Accession # NP_001028715	KDNIEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001028715
• N-terminal Sequence: Asp14 & Val16
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il20rb
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 49.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-66 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-20 R beta Fc Chimera Protein, CF

• FNDC6
• DIRS1
• FNDC6
• IL-20 R beta
• IL20R beta
• IL-20R2
• IL20RB interleukin 20 receptor beta
• IL20RB
• IL-20Rb
• MGC34923

Background

IL-20 receptor beta (IL-20 R beta ), also known as IL-20 R2, is a type I transmembrane glycoprotein in the class II cytokine receptor family. These receptors are characterized by tandem fibronectin type III domains in their extracellular region and the lack of a WSXWS motif (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). Based on the structure of the human receptor, mouse IL-20 R beta should contain a 202 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains, a 16 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, mouse IL-20 R beta will share 81% aa sequence identity with human IL-20 R beta . It shares 18%‑29% aa sequence identity with the mouse class II cytokine receptors IFN-alpha R1, IFN-alpha R2, IFN-gamma R1, IFN-gamma R2, IL-10 R alpha , IL-10 R beta , IL-20 R alpha , IL-22BP, IL-22 R alpha , IL-28 R, and tissue factor. IL-20 R beta is generally expressed in psoriatic skin (keratinocytes and select endothelium), rheumatoid arthritis synovial membranes, and hepatocytes of LPS-treated mice, and it contributes to the local inflammatory reaction (3‑6). IL-20 R beta heterodimerizes with IL-20 R alpha to form the receptor complex that mediates IL-19, IL-20 and IL-24 signals (3, 7‑10). It also heterodimerizes with IL-22 R to form the functional receptor complex for IL-20 and IL-24 (7‑9). Binding of these IL-10 family class II cytokines to their receptors induces activation of the JAK-STAT signal transduction pathway.

1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
2. Accession # NP_001032323 and SwissProt # Q6UXL0.
3. Blumberg, H. et al. (2001) Cell 104:9. 
4. Hsu, Y.-H. et al. (2006) Arthritis Rheum. 54:2722. 
5. Wegenka, U.M. et al. (2007) J. Hepatol. 46:257. 
6. Chan, J.R. et al. (2006) J. Exp. Med. 203:2577.
7. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
8. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
9. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
10. Pletnev, S. et al. (2003) Biochemistry 42:12617.

Publications for IL-20 R beta/FNDC6 (4388-MR)(2)

Publications using 4388-MR

• M Tsubaki, T Takeda, T Matsuda, Y Yamamoto, A Higashinak, K Yamamoto, K Tsurushima, T Ishizaka, S Nishida Interleukin 19 suppresses RANKL-induced osteoclastogenesis via the inhibition of NF-&amp;kappaB and p38MAPK activation and c-Fos expression in RAW264.7 cells Cytokine, 2021-05-27;144(0):155591. 2021-05-27 [PMID: 34052656] (Bioassay, Mouse)
• Uto-Konomi A, Miyauchi K, Ozaki N Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines. PLoS ONE, 2012-07-05;7(7):e40343. 2012-07-05 [PMID: 22792286] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: Il20rb
• Uniprot:
  • NP_001028715()