Recombinant Mouse IL-13 R alpha 2 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-13 R alpha 2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-13-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.01‑0.03 µg/mL in the presence of 8 ng/mL of recombinant mouse IL-13.
• Source: Mouse myeloma cell line, NS0-derived mouse IL-13 R alpha 2 protein
  

  Mouse IL-13 R alpha 2 (Leu22 - Lys334) Accession # O88786	IEGRDID	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: O88786
• N-terminal Sequence: Leu22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il13ra2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 63 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-13 R alpha 2 Fc Chimera Protein, CF

• cancer/testis antigen 19
• CD213a2 antigen
• CD213a2
• CT19
• IL-13 R alpha 2
• IL-13 receptor subunit alpha-2
• IL13BP
• IL13R alpha 2
• IL-13R subunit alpha-2
• IL13R
• IL-13R
• IL13RA2
• IL-13Ra2
• IL-13R-alpha-2
• interleukin 13 binding protein
• interleukin 13 receptor alpha 2 chain
• interleukin 13 receptor, alpha 2
• interleukin-13 receptor subunit alpha-2
• Interleukin-13-binding protein

Background

Interleukin-13 Receptor alpha 2 (IL-13 Ra2), also known as IL-13 Ra’, IL-13 binding protein, and CD213a2, is a widely expressed 55 kDa cytokine receptor that plays an important role in the Th2‑polarized immune responses characteristic of a variety of pathologies including parasitic infections and allergic asthma (1, 2). Mature mouse IL-13 Ra2 consists of a 313 amino acid (aa) extracellular domain with three fibronectin type-III domains, a WSxWS motif, a 21 aa transmembrane segment, and a 28 aa cytoplasmic domain (3). Within the ECD, mouse IL-13 Ra2 shares 64% and 94% aa sequence identity with human and rat IL-13 Ra2, respectively. A 40 kDa ‑ 50 kDa soluble form of mouse IL-13 Ra2 can be generated by alternate splicing or MMP-8 mediated shedding (4, 5). The biological effects of IL-13 and IL-4 are closely related in part due to a shared receptor system. IL‑13 binds to IL-13 Ra1 which then forms a signaling complex with IL-4 Ra (6, 7). IL-13 Ra2 functions as a decoy receptor by binding and internalizing IL-13 and preventing it from signaling through the IL-13 Ra1/IL-4 Ra complex (3, 8). IL‑13 Ra2 can also block IL-4 induced responses by inhibiting IL-4 bound IL-13 Ra1/IL-4 Ra receptor complexes even though it does not itself bind IL-4 (9, 10). Aside from its decoy function, IL‑13‑activated IL-13 Ra2 directly promotes the development of tissue fibrosis by inducing the transcription of TGF-beta (11). Soluble IL‑13 Ra2 retains ligand binding capability and attenuates responses to IL-13 but not to IL-4 (9, 12). The up‑regulation of transmembrane and soluble IL‑13 Ra2 during Th2-biased immune responses limits the extent of those responses (13 ‑ 15). IL‑13 Ra2 is expressed in some cancers, and its ability to block IL-13 and IL-4 contributes to tumorigenesis and metastasis (10, 16).

1. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
2. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
3. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
4. Tabata, Y. et al. (2006) J. Immunol. 177:7905.
5. Chen, W. et al. (2008) J. Allergy Clin. Immunol. 122:625.
6. Andrews, A.-L. et al. (2006) J. Immunol. 176:7456.
7. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
8. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
9. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
10. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
11. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.
12. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
13. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
14. Morimoto, M. et al. (2009) J. Immunol. 183:1934.
15. Zheng, T. et al. (2008) J. Immunol. 180:522.
16. Fujisawa, T. et al. (2009) Cancer Res. 69:8678.

Publications for IL-13R alpha 2 (539-IR)(11)

Publications using 539-IR

• M Álvarez-Iz, M Pérez-Cres, JG Esteban, C Muñoz-Anto, R Toledo Interleukin-25-mediated resistance against intestinal trematodes does not depend on the generation of Th2 responses Parasites & vectors, 2020-12-04;13(1):608. 2020-12-04 [PMID: 33276813] (In Vivo, Mouse)
• Lee , Gerald B, Brandt , Eric B, Xiao , Chang, Gibson , Aaron M, Le Cras , Timothy, Brown , Lou Ann, Fitzpatrick , Anne M, Khurana Hershey , Gurjit K Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma. PLoS ONE, 2013-03-29;8(3):e60632. 2013-03-29 [PMID: 23555996] (Bioassay, Mouse)
• Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK A novel combination immunotherapy for cancer by IL-13Ralpha2-targeted DNA vaccine and immunotoxin in murine tumor models. J. Immunol., 2011-10-17;187(10):4935-46. 2011-10-17 [PMID: 22013118] (ELISA (Capture), Mouse)
• Deepak P, Acharya A Interleukin-13 induces T helper type 2 immune responses in OVA-immunized BALB/c mice bearing a T cell lymphoma. Scand. J. Immunol., 2012-01-01;75(1):85-95. 2012-01-01 [PMID: 21923743] (In Vivo, Mouse)
• Deepak P, Kumar S, Kishore D, Acharya A IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma. Int. Immunol., 2009-11-30;22(1):53-63. 2009-11-30 [PMID: 19951958] (Bioassay, In Vivo, Mouse)
• Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo. J. Allergy Clin. Immunol., 2008-08-09;122(3):625-32. 2008-08-09 [PMID: 18694590] (ELISA (Standard))
• Matsumoto K, Fukuyama S, Eguchi-Tsuda M, Nakano T, Matsumoto T, Matsumura M, Moriwaki A, Kan-o K, Wada Y, Yagita H, Shin T, Pardoll DM, Patcharee R, Azuma M, Nakanishi Y, Inoue H B7-DC induced by IL-13 works as a feedback regulator in the effector phase of allergic asthma. Biochem. Biophys. Res. Commun., 2007-11-05;365(1):170-5. 2007-11-05 [PMID: 17981145] (In Vivo, Mouse)
• Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice. J. Clin. Invest., 2007-10-01;117(10):2941-51. 2007-10-01 [PMID: 17885690] (ELISA (Standard))
• Hayashi N, Yoshimoto T, Izuhara K, Matsui K, Tanaka T, Nakanishi K T helper 1 cells stimulated with ovalbumin and IL-18 induce airway hyperresponsiveness and lung fibrosis by IFN-gamma and IL-13 production. Proc. Natl. Acad. Sci. U.S.A., 2007-08-31;104(37):14765-70. 2007-08-31 [PMID: 17766435] (In Vivo, Mouse)
• Deepak P, Kumar S, Acharya A Interleukin-13-induced type II polarization of inflammatory macrophages is mediated through suppression of nuclear factor-kappaB and preservation of IkappaBalpha in a T cell lymphoma. Clin. Exp. Immunol., 2007-06-05;149(2):378-86. 2007-06-05 [PMID: 17550372] (In Vivo, Mouse)
• Kawashima R, Kawamura YI, Kato R, Mizutani N, Toyama-Sorimachi N, Dohi T IL-13 receptor alpha2 promotes epithelial cell regeneration from radiation-induced small intestinal injury in mice. Gastroenterology, 2006-07-01;131(1):130-41. 2006-07-01 [PMID: 16831597] (ELISA (Standard))

Bioinformatics

• Gene Symbol: Il13ra2
• Uniprot:
  • O88786()