Recombinant Mouse IGFBP-L1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IGFBP-L1 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Cai, Z. et al. (2005) BBRC 331:261. The ED₅₀ for this effect is 2-8 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse IGFBP-L1 protein
  Leu18-Leu270, with a C-terminal 10-His tag
• Accession #: Q80W15
• N-terminal Sequence: Leu18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Igfbpl1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 28.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IGFBP-L1 Protein, CF

• bA113O24.1
• IGFBPL1 insulin-like growth factor binding protein-like 1
• IGFBPL1
• IGFBP-L1
• IGFBP-RP4

Background

Insulin-like Growth Factor Binding Protein-Like 1, also known as IGFBP-rP4, is a 38 kDa secreted member of the IGFBP superfamily of proteins. IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins (1). IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region, and a C-terminal Ig-like domain (2, 3). Mature mouse IGFBP-L1 shares 76% and 96% amino acid (aa) sequence identity with human and rat IGFBP-L1, respectively. It shares 38% - 41% aa sequence identity with mouse IGFBP-7 and IGFBP-rp10 and 20% - 24% aa sequence identity with mouse IGFBP-1, -2, -3, -4, -5, and -6. IGFBP-L1 is most highly expressed in testis and brain, while lower levels are more broadly expressed (3). IGFBP-L1 is expressed in regionally and temporally distinct patterns during forebrain development (4). IGFBP-L1 inhibits cell proliferation in vitro and is downregulated in many colon and lung tumors (3). Transcriptional downregulation by promoter methylation is predictive of breast cancer aggressiveness (5).

1. Firth, S.M. and R.C. Baxter (2002) Endocr. Rev. 23:824.
2. Accession # Q80W15.
3. Cai, Z. et al. (2005) Biochem. Biophys. Res. Commun. 331:261.
4. Gonda, Y. et al. (2007) Gene Expr. Patterns 7:431.
5. Smith, P. et al. (2007) Clin. Cancer Res. 13:4061.

Publications for IGFBP-L1 (4130-BL)(2)

Publications using 4130-BL

• Pan, L;Cho, KS;Wei, X;Xu, F;Lennikov, A;Hu, G;Tang, J;Guo, S;Chen, J;Kriukov, E;Kyle, R;Elzaridi, F;Jiang, S;Dromel, PA;Young, M;Baranov, P;Do, CW;Williams, RW;Chen, J;Lu, L;Chen, DF; IGFBPL1 is a master driver of microglia homeostasis and resolution of neuroinflammation in glaucoma and brain tauopathy Cell reports 2023-07-31 [PMID: 37527036] (Cell Culture)
• E Butti, S Cattaneo, M Bacigalupp, M Cambiaghi, GM Scotti, E Brambilla, F Ruffini, G Sferruzza, M Ripamonti, F Simeoni, L Cacciaguer, A Zanghì, A Quattrini, R Fesce, P Panina-Bor, F Giannese, D Cittaro, T Kuhlmann, P D'Adamo, MA Rocca, S Taverna, G Martino Neural precursor cells tune striatal connectivity through the release of IGFBPL1 Nature Communications, 2022-12-08;13(1):7579. 2022-12-08 [PMID: 36482070] (ELISA Standard, Mouse)

Bioinformatics

• Gene Symbol: Igfbpl1
• Uniprot:
  • Q80W15()