Recombinant Mouse IGFBP-L1 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IGFBP-L1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Cai, Z. et al. (2005) BBRC 331:261. The ED50 for this effect is 2-8 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IGFBP-L1 protein
Leu18-Leu270, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Leu18
Protein/Peptide Type
Recombinant Proteins
Gene
Igfbpl1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
28.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IGFBP-L1 Protein, CF

  • bA113O24.1
  • IGFBPL1 insulin-like growth factor binding protein-like 1
  • IGFBPL1
  • IGFBP-L1
  • IGFBP-RP4

Background

Insulin-like Growth Factor Binding Protein-Like 1, also known as IGFBP-rP4, is a 38 kDa secreted member of the IGFBP superfamily of proteins. IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins (1). IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region, and a C-terminal Ig-like domain (2, 3). Mature mouse IGFBP-L1 shares 76% and 96% amino acid (aa) sequence identity with human and rat IGFBP-L1, respectively. It shares 38% - 41% aa sequence identity with mouse IGFBP-7 and IGFBP-rp10 and 20% - 24% aa sequence identity with mouse IGFBP-1, -2, -3, -4, -5, and -6. IGFBP-L1 is most highly expressed in testis and brain, while lower levels are more broadly expressed (3). IGFBP-L1 is expressed in regionally and temporally distinct patterns during forebrain development (4). IGFBP-L1 inhibits cell proliferation in vitro and is downregulated in many colon and lung tumors (3). Transcriptional downregulation by promoter methylation is predictive of breast cancer aggressiveness (5).

  1. Firth, S.M. and R.C. Baxter (2002) Endocr. Rev. 23:824.
  2. Accession # Q80W15.
  3. Cai, Z. et al. (2005) Biochem. Biophys. Res. Commun. 331:261.
  4. Gonda, Y. et al. (2007) Gene Expr. Patterns 7:431.
  5. Smith, P. et al. (2007) Clin. Cancer Res. 13:4061.

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Bioinformatics

Gene Symbol Igfbpl1
Uniprot