Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF

Images

 
2 μg/lane of Recombinant Mouse IFN-alpha J1/IFNA7 (Catalog # 10208-IJ) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ...read more
Recombinant Mouse IFN-alpha 7/IFNA7 (Catalog # 10208-IJ) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <1.5 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF Summary

Details of Functionality
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is <1.5 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse IFN-alpha 7/IFNA7 protein
Cys24-Glu190
Accession #
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-24 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF

  • IFNA7
  • IFN-alpha 7
  • IFNalpha J1
  • IFN-alpha J1
  • interferon alpha-7
  • interferon alpha-J1
  • interferon, alpha 7

Background

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). Mature mouse IFNA7 consists of 166 aa and shares 59% identity with human IFNA7. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection, with IFNA7 showing slightly lower antiviral potency against Mengo virus but maintaining similar anti-proliferative potency in B16 melanoma cells (5, 6).
  1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
  2. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  3. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  4. Fung, K.Y. et al. (2013) Science 339:1088.
  5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  6. James CM et al. (2007) Vaccine. 25(10):1856.

Customers Who Viewed This Item Also Viewed...

NBP2-75930
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-381
Species: Hu
Applications: WB, ChIP, IP
NBP2-67634
Species: Hu, Mu, Rt, Fi, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB500-487
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-04606
Species: Hu
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-31207
Species: Hu, Mu, Rt, Po, Sq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-326
Species: NA
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-67330
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB7276
Species: Ch
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-67741
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-74124
Species: Hu, Po, Bv, Ca, Gt, Sh
Applications: WB

Publications for IFN-alpha J1/IFNA7 (10208-IJ) (0)

There are no publications for IFN-alpha J1/IFNA7 (10208-IJ).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IFN-alpha J1/IFNA7 (10208-IJ) (0)

There are no reviews for IFN-alpha J1/IFNA7 (10208-IJ). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IFN-alpha J1/IFNA7 (10208-IJ) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IFN-alpha J1/IFNA7 Products

Pathways for IFN-alpha J1/IFNA7 (10208-IJ)

View related products by pathway.

Blogs on IFN-alpha J1/IFNA7

There are no specific blogs for IFN-alpha J1/IFNA7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot