Recombinant Mouse FcgR4/CD16-2 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse FcgR4/CD16-2 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind mouse IgG with an estimated K_d <1 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse FcgR4/CD16-2 protein
  Gly21-Gln203, with a C-terminal 10-His tag
• Accession #: NP_653142
• N-terminal Sequence: Gly21
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fcgr4
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 22.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25-35 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FcgR4/CD16-2 Protein, CF

• 4833442P21Rik;CD16-2;Fcgam;FcgammaRIV;Fcgr;Fcgr3a;FcgRIV;Fcrl3;Low affinity immunoglobulin gamma Fc region receptor III-A
• CD16-2
• FcgR4
• Fcrl3

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10^-8-10^-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10^-6-10^-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Human CD16 is encoded by two genes; Fc gamma RIIIA is an activating transmembrane receptor expressed in macrophages and NK cells, and Fc gamma RIIIB is a GPI-linked protein on granulocytes (2). Mouse Fc gamma RIII and human Fc gamma RIIIA share 44% amino acid identity and are frequently described as functional equivalents (5).

CD16-2, also referred to as Fcg R4, is a protein that is more closely related to human Fc gamma  RIIIA (60% amino acid identity) and may be its true ortholog (6). Similar to other activating receptors, it possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM bearing accessory protein. CD16-2 RNA expression was observed in peripheral blood leukocytes, spleen, thymus, colon, and intestine.

1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.  
3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275. 
4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
5. Ravetch, J. and L. Lanier (2000) Science 290:84.
6. Mechetina, L. et al. (2002) Immunogenetics 54:463.

Publications for FcgR4/CD16-2 (1974-CD)(1)

Publication using 1974-CD

• Hirano M, Davis RS, Fine WD, Nakamura S, Shimizu K, Yagi H, Kato K, Stephan RP, Cooper MD IgEb immune complexes activate macrophages through FcgammaRIV binding. Nat. Immunol., 2007-06-10;8(7):762-71. 2007-06-10 [PMID: 17558411] (Surface Plasmon Resonance, Human, Mouse)

Bioinformatics

• Gene Symbol: Fcgr4
• Uniprot:
  • NP_653142()