Recombinant Mouse DPPIV/CD26 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Mouse DPPIV/CD26 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >2,500 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived mouse DPPIV/CD26 protein
Ser29-His760, with a C-terminal 9-His tag
Accession #
N-terminal Sequence
Ser29
Protein/Peptide Type
Recombinant Enzymes
Gene
Dpp4
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
105 kDa, reducing conditions
Publications
Read Publications using
954-SE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 25 mM Tris, pH 8.0
  • Recombinant Mouse DPPIV/CD26 (rmCD26) (Catalog # 954-SE)
  • Substrate: Gly-Pro-AMC (Bachem, Catalog # I-1225), 10 mM stock in DMSO.
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rmCD26 to 0.2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 40 µM in Assay Buffer.
  3. In a plate load 50 µL of 0.2 ng/µL rmCD26 and start the reaction by adding 50 µL of 40 µM Substrate. Include a Substrate blank containing 50 µL Assay Buffer and 50 µL of Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).

Per Well:
  • rmCD26: 0.010 µg
  • Substrate: 20 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse DPPIV/CD26 Protein, CF

  • ADABP
  • ADCP-2
  • ADCP2DPP IV
  • Adenosine deaminase complexing protein 2TP103
  • CD26 antigen
  • CD26
  • CD26T-cell activation antigen CD26
  • dipeptidyl peptidase 4
  • Dipeptidyl peptidase IV
  • dipeptidylpeptidase 4
  • dipeptidyl-peptidase 4
  • dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
  • DPP4
  • DPPIV
  • EC 3.4.14.5

Background

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

  1. Misumi, Y. and Y. Ikehara (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds), p. 1905, Elsevier, London.
  2. Ikehara, Y. et al. (1994) Methods Enzymol. 244:215.
  3. Marguet, D. et al. (1992) J. Biol. Chem. 267:2200.
  4. Bernard, A.M. et al. (1994) Biochemistry 33:15204.
  5. Vivier, I. et al. (1991) J. Immunol. 147:447.
  6. Callebaut, C. et al. (1993) Science 262:2045.
  7. Kameoka, J. et al. (1993) Science 261:466.
  8. Ohtsuki, T. et al. (1998) FEBS Lett. 431:236.
  9. Proost, P. et al. (1999) J. Biol. Chem. 274:3988.
  10. Hinke, S.A. et al. (2000) J. Biol. Chem. 275:3827.
  11. Wrenger, S. et al. (2000) FEBS Lett. 466:155.

Publications for DPPIV/CD26 (954-SE)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Bioassay, Enzyme Assay.


Filter By Application
Bioassay
(3)
Enzyme Assay
(1)
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Mouse
(5)
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Showing Publications 1 - 5 of 5.
Publications using 954-SE Applications Species
TD Madsen, LH Hansen, J Hintze, Z Ye, S Jebari, DB Andersen, HJ Joshi, T Ju, JP Goetze, C Martin, MM Rosenkilde, JJ Holst, RE Kuhre, CK Goth, SY Vakhrushev, KT Schjoldage An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles Nat Commun, 2020-08-20;11(1):4033. 2020-08-20 [PMID: 32820167] (Bioassay, Mouse) Bioassay Mouse
C Baumeier, L Schlüter, S Saussentha, T Laeger, M Rödiger, SA Alaze, L Fritsche, HU Häring, N Stefan, A Fritsche, RW Schwenk, A Schürmann Elevated hepatic DPP4 activity promotes insulin resistance and non-alcoholic fatty liver disease Mol Metab, 2017-08-04;6(10):1254-1263. 2017-08-04 [PMID: 29031724] (Bioassay, Mouse) Bioassay Mouse
TH Ambrosi, A Scialdone, A Graja, S Gohlke, AM Jank, C Bocian, L Woelk, H Fan, DW Logan, A Schürmann, LR Saraiva, TJ Schulz Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration Cell Stem Cell, 2017-03-16;20(6):771-784.e6. 2017-03-16 [PMID: 28330582] (Mouse) Mouse
Pang , Zhengda, Nakagami , Hironori, Osako , Mariana, Koriyama , Hiroshi, Nakagami , Futoshi, Tomioka , Hideki, Shimamura , Munehisa, Kurinami , Hitomi, Takami , Yoichi, Morishita , Ryuichi, Rakugi , Hiromi Therapeutic vaccine against DPP4 improves glucose metabolism in mice. Proc Natl Acad Sci U S A, 2014-03-17;111(13):E1256-63. 2014-03-17 [PMID: 24639549] (Bioassay, Mouse) Bioassay Mouse
Yasuda N, Nagakura T, Inoue T, Yamazaki K, Katsutani N, Takenaka O, Clark R, Matsuura F, Emori E, Yoshikawa S, Kira K, Ikuta H, Okada T, Saeki T, Asano O, Tanaka I E3024, 3-but-2-ynyl-5-methyl-2-piperazin-1-yl-3,5-dihydro-4H-imidazo[4,5-d]pyridazin-4-one tosylate, is a novel, selective and competitive dipeptidyl peptidase-IV inhibitor. Eur. J. Pharmacol., 2006-08-16;548(1):181-7. 2006-08-16 [PMID: 16973152] (Enzyme Assay, Mouse) Enzyme Assay Mouse

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Bioinformatics

Gene Symbol Dpp4
Uniprot