Recombinant Mouse CL-P1/COLEC12 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse CL-P1/COLEC12 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmCL-P1 at 5 µg/mL (100 µL/well) can bind biotinylated advance glycation endproducts of bovine serum albumin (AGE-BSA) with a linear range of
  3-200 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CL-P1/COLEC12 protein
  Ala101-Leu742, with an N-terminal 6-His tag
• Accession #: Q8K4Q8
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: Colec12
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 118-133 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CL-P1/COLEC12 Protein, CF

• CL-P1
• CLP1hCL-P1
• COLEC12
• collectin placenta 1
• Collectin placenta protein 1
• collectin sub-family member 12
• collectin-12
• NSR2
• Nurse cell scavenger receptor 2
• SCARA4
• SCARA4NSR2
• Scavenger receptor class A member 4
• scavenger receptor class A, member 4
• SRCL Type I
• SRCLScavenger receptor with C-type lectin

Background

Collectins are a family of Ca⁺⁺-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1 - 4). Collectin placenta 1 (CL-P1), also known as collectin sub-family member 12 and scavenger receptor with C-type lectin type I (SRCL), is a 140 kDa member of the collectin family of glycoproteins. With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Mouse CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that includes an N-terminal 39 aa cytoplasmic domain, an 18 aa transmembrane segment, and a 685 aa C-terminal extracellular domain. The short cytoplasmic domain contains an internalization motif (Y-K-R-F), while the ECD is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure, and a C-type lectin/carbohydrate recognition domain (CRD) (5, 6). Unlike human CL-P1, no splice variants of mouse CL-P1 have been described (5, 7). Trimerization of CL-P1 is mediated by its collagen-like and coiled-coil helical domains (1, 6). Within the ECD, mouse CL-P1 shares 88%, 89%, 92%, and 98% aa sequence identity with bovine, canine, human, and rat CL-P1, respectively. The CRD shares 23 - 27% aa sequence identity with the CRD of collectins CL-L1, collectin sub-family member 11, MBL, SP-A1, and SP-D. Notably, this CRD recognizes galactose and fucose within the context of asialo-orosomucoids associated with the Lewis^x epitope (8, 9). CL-P1 is expressed in vascular endothelial cells and may play a role in bacterial recognition or as a scavenger receptor for desialylated glycoproteins (6, 8).

1. van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
5. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
6. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
7. Nakamura, K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
8. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
9. Yoshida, T. et al. (2003) J. Biochem. 133:271.

Publications for CL-P1/COLEC12 (3130-CL)(1)

Publication using 3130-CL

• K Grabert, P Engskog-Vl, M Škandík, G Vazquez-Ca, AN Murgoci, L Keane, M Gaetani, B Joseph, M Cheray Proteome integral solubility alteration high-throughput proteomics assay identifies Collectin-12 as a non-apoptotic microglial caspase-3 substrate Cell Death & Disease, 2023-03-11;14(3):192. 2023-03-11 [PMID: 36906641] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Colec12
• Uniprot:
  • Q8K4Q8()