Recombinant Mouse CL-K1/COLEC11 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse CL-K1/COLEC11 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind alpha -L-Fucose. Keshi, H. et al. (2006) Microbiol. Immunol. 50(12):1001.
• Source: Mouse myeloma cell line, NS0-derived mouse CL-K1/COLEC11 protein
  Gln27-Leu272, with a C-terminal 6-His tag
• Accession #: Q3SXB8
• N-terminal Sequence: No results obtained: Gln27 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: Colec11
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 33-38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CL-K1/COLEC11 Protein, CF

• CLK1
• CL-K1
• CL-K1-I
• CL-K1-II
• CL-K1-IIa
• CL-K1-IIb
• COLEC11
• collectin kidney I
• Collectin kidney protein 1
• collectin sub-family member 11
• collectin-11
• DKFZp686N1868
• MGC3279

Background

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the serum (4, 5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23% - 32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

1. Gupta, G. and A. Surolia (2007) Bioessays 29:452.
2. van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
3. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
4. Keshi, H. et al. (2006) Microbiol. Immunol. 50:1001.
5. Accession # NP_082142.

Bioinformatics

• Gene Symbol: Colec11
• Uniprot:
  • Q3SXB8()