Recombinant Mouse CDCP1 Protein, CF

• Reactivity: Mu
• Format: Carrier-Free

Recombinant Mouse CDCP1 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse CDCP1 protein
  Arg25-Ala667, with a C-terminal 6-His tag
• Accession #: Q5U462
• N-terminal Sequence: Arg25 & Ser30
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-108 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CDCP1 Protein, CF

• CD318 antigen
• CD318
• CDCP1
• CUB domain containing protein 1
• CUB domain-containing protein 1
• Membrane glycoprotein gp140
• SIMA135
• SIMA135TRASK
• Subtractive immunization M plus HEp3-associated 135 kDa protein
• Transmembrane and associated with src kinases

Background

CDCP1 (CUB-domain containing protein 1; also known as CD318 and SIMA135) is a novel, 135 kDa cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells (1). It is reported that this protein is over-expressed in colon and lung cancers. CDCP1 is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa 30‑667) and a phosphotyrosine site at Tyr731. The phosphorylation state of CDCP-1 has an effect on anchorage in epithelial cells (2). When unligated, CDCP1 can be proteolytically cleaved between aa 270‑300. This generates an 80 kDa TM protein that may be missing the N-terminal CUB domain (aa 221‑350) (3). Over aa 25‑667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.

1. Hooper, J.D. et al. (2003) Oncogene 22:1783.
2. Spassov, D.S. et al. (2013) Cancer Res. 73:1168.
3. He, Y. et al. (2010) J. Biol. Chem. 285:26162.

Publications for CDCP1 (9688-CU)(1)

Publication using 9688-CU

• L Zhang, N Borjini, Y Lun, S Parab, G Enyindah-A, R Singh, BA Bell, VL Bonilha, AI Ivanov, DA Fox, RR Caspi, F Lin CDCP1 regulates retinal pigmented epithelial barrier integrity for the development of experimental autoimmune uveitis JCI Insight, 2022-09-22;0(0):. 2022-09-22 [PMID: 35951427] (In Vivo, Mouse)

Bioinformatics

• Uniprot:
  • Q5U462()