Recombinant Mouse CD2F-10/SLAMF9 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD2F-10/SLAMF9 Protein, CF Summary

• Details of Functionality: Meassure by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3⁺ T cells. The ED₅₀ for this effect is 0.4‑2 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CD2F-10/SLAMF9 protein
  Phe18-Leu230, with a C-terminal 6-His tag
• Accession #: Q9D780
• N-terminal Sequence: Phe18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Slamf9
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD2F-10/SLAMF9 Protein, CF

• CD2 family member 10
• CD2F10
• CD2F-10
• CD2F-10MGC88194
• CD84 homolog 1
• CD84H1
• CD84-H1
• CD84-H1cluster of differentiation 2 antigen family member 10
• cluster of differentiation 84 homolog 1
• SF2001
• signaling lymphocytic activation molecule family member 2001
• signaling lymphocytic activation molecule family member 9
• SLAM family member 9
• SLAMF9

Background

The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F‑10 protein, also known as SF2001 and CD85‑H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F‑10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co‑receptors for lymphocyte activation and/or adhesion (1). Mature mouse CD2F‑10 is a type I transmembrane glycoprotein that consists of a 215 amino acid (aa) extracellular domain (ECD) with one Ig‑like V‑set and one Ig‑like C2‑set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2 ‑ 4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F‑10 does not contain consensus immunoreceptor tyrosine‑based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT‑2 (1). Within the ECD, mouse CD2F‑10 shares 58% and 83% aa sequence identity with human and rat CD2F‑10, respectively. It shares 21% ‑ 38% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD48, CD84, CD229, CRACC, NTB‑A, and SLAM. CD2F‑10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F‑10 costimulates the secretion of IFN‑ gamma from activated mouse T cells.

1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol.  29:665.
2. Fennelly, J.A. et al. (2001) Immunogenetics 53:599.
3. Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.
4. Fraser, C.C. et al. (2002) Immunogenetics 53:843.

Bioinformatics

• Gene Symbol: Slamf9
• Uniprot:
  • Q9D780()