Recombinant Mouse CD200R1 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse CD200R1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 R1 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) that produces 50% of the optimal binding response is appoximately 5-30 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CD200R1 protein
  

  Mouse CD200 R1 (Thr26-Pro238) Accession # BAC40777.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: BAC40777.1
• N-terminal Sequence: Thr26
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd200r1
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 49.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD200R1 Fc Chimera Protein, CF

• CD200 R1
• CD200 receptor 1
• CD200R1
• CD200RMOX2Rcell surface glycoprotein CD200 receptor 1
• Cell surface glycoprotein OX2 receptor 1
• cell surface glycoprotein receptor CD200
• CRTR2
• HCRTR2
• MOX2 receptor
• MOX2R
• OX2RCD200 cell surface glycoprotein receptor

Background

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1-3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5-7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13-15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. 
3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. 
4. Wright, G.J. et al. (2003) J. Immunol. 171:3034. 
5. Shiratori, I. et al. (2005) J. Immunol. 175:4441. 
6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. 
7. Fallarino, F. et al. (2004) J. Immunol. 173:3748. 
8. Wright, G.J. et al. (2001) Immunology 102:173. 
9. Hoek, R.M. et al. (2000) Science 290:1768.
10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
13. Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.
14. Zhang, S. et al. (2004) J. Immunol. 173:6786.
15. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
16. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.

Publications for CD200R1 (2554-CD)(1)

Publication using 2554-CD

• Yi M, Zhang E, Kim J, Baek H, Shin N, Kim S, Kim S, Kim H, Lee S, Park J, Kim Y, Kwon O, Lee Y, Oh S, Kim D CD200R/Foxp3-mediated signalling regulates microglial activation. Sci Rep, 2016-10-12;6(0):34901. 2016-10-12 [PMID: 27731341] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Cd200r1
• Uniprot:
  • BAC40777.1()