NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD200 R1 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD200 R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2554) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 6-7 days were stained with Goat Anti-Human CD200 R1 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF3414, filled histogram) or isotype control (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107)." class="big_lightbox"> 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 6-7 days were stained with Goat Anti-Human CD200 R1 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF3414, filled histogram) or isotype control (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107)." alt="Human immature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14⁺cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 6-7 days were stained with Goat Anti-Human CD200 R1 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF3414, filled histogram) or isotype control (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107)." class="big_thumb" />

F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0102B). View our protocol for Staining Membrane-associated Proteins. " alt="HEL-92 (filled histogram) vs Jurkat cells (open histogram) were stained with Mouse Anti-Human CD200R1 Monoclonal Antibody (Catalog # MAB3414) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />