Recombinant Mouse BTNL2/Butyrophilin-like 2 Protein, CF

Recombinant Mouse BTNL2/Butyrophilin-like 2 inhibits IL-2secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50for this effect is 1-6 ug/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse BTNL2/Butyrophilin-like 2 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 1-6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse BTNL2/Butyrophilin-like 2 protein
  Asp27-Ser452, with a C-terminal 6-His tag
• Accession #: O70355
• N-terminal Sequence: Asp27
• Protein/Peptide Type: Recombinant Proteins
• Gene: Btnl2
• Purity: >95%, by SDS-PAGE with silver staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-67 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BTNL2/Butyrophilin-like 2 Protein, CF

• BTL-II
• BTNL2
• Butyrophilin like 2
• butyrophilin-like 2 (MHC class II associated)
• Butyrophilin-like 2

Background

Butyrophilin-like 2 (BTNL2) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1).Mouse BTNL2 is a 514 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD features two V-type Ig-like domains, two C-type Ig-like domains, and four glycosylation sites. The ECD of mouse BTNL2 (aa 27-452) shares 64% and 88% sequence identity with the ECD of human and rat BTNL2, respectively. A splice variant of BTNL2 lacks the second Ig-like domain in the ECD (2). BTNL2 is expressed in epithelial cells of the small intestine, colonic dendritic cells, and in cells of the lymph node (1, 2). BTNL2 expression is upregulated in T cells following activation, a characteristic BTNL2 shares with the homologous B7 family of co-stimulatory molecules (3, 4). BTNL2 negatively regulates T cells by inhibiting proliferation and inflammatory cytokine secretion (1, 3). It also increases the expression of FoxP3 in T cells to promote regulatory T cell development (5). Single nucleotide polymorphisms in BTNL2 are associated with a risk for sporadic prostate cancer, rheumatoid arthritis, sarcoidosis, ulcerative colitis, and other inflammatory diseases (2, 6-12).

1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
2. Arnett, H.A. et al. (2009) Cytokine 46:370.
3. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
4. Nguyen, T. et al. (2006) J. Immunol. 176:7354.
5. Swanson, R.M. et al. (2013) J. Immunol. 190:2027.
6. Morais, A. et al. (2012) Respir. Med. 106:1771.
7. Milman, N. et al. (2011) Clin. Respir. J. 5:105.
8. Wijnen, P.A. et al. (2011) Hum. Immunol. 72:342.
9. Valentonyte, R. et al. (2005) Nat. Genet. 37:357.
10. Pathan, S. et al. (2009) Tissue. Antigens 74:322.
11. Mitsunaga, S. et al. (2013) J. Hum. Genet. 58:210.
12. Fitzgerald, L.M. et al. (2013) Cancer Epidemiol. Biomarkers Prev. 22:1520.

Bioinformatics

• Gene Symbol: Btnl2
• Uniprot:
  • O70355()