HAF019). A specific band was detected for BTNL2/Butyrophilin-like 2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse BTNL2/Butyrophilin-like 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BTNL2/Butyrophilin-like 2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5236-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92488-0.02ml | |||
![Western Blot: BTNL2/Butyrophilin-like 2 Antibody - Azide and BSA Free [NBP2-92488] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BTNL2/Butyrophilin-like 2 using BTNL2/Butyrophilin-like 2 Rabbit pAb at 1:3000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Enhanced Kit.<br>Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp2-92488_rabbit-polyclonal-btnl2-butyrophilin-like-2-antibody-42202514275226.jpg "Western Blot: BTNL2/Butyrophilin-like 2 Antibody - Azide and BSA Free [NBP2-92488] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BTNL2/Butyrophilin-like 2 using BTNL2/Butyrophilin-like 2 Rabbit pAb at 1:3000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Enhanced Kit.<br>Exposure time: 60s.")
![Immunohistochemistry: BTNL2/Butyrophilin-like 2 Antibody - Azide and BSA Free [NBP2-92488] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using BTNL2/Butyrophilin-like 2 Rabbit pAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92488_rabbit-polyclonal-btnl2-butyrophilin-like-2-antibody-4220251429010.jpg)
